|Table of Contents|

Isolation,Identification and Pathogenicity of Pathogens Causing Gerbera Root Rot Disease

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年14
Page:
87-93
Research Field:
Publishing date:

Info

Title:
Isolation,Identification and Pathogenicity of Pathogens Causing Gerbera Root Rot Disease
Author(s):
HE Haiyong1; ZHAO Dailin1; TAN Qingqun1; YANG Xuehui1; SHI Lejuan2
(1.Institute of Plant Protection,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006;2.Horticultural Institute,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006)
Keywords:
gerbera; root rot; morphological identification; molecular identification; pathogenicity test
PACS:
-
DOI:
10.11937/bfyy.20205507
Abstract:
The disease plants of gerbera root rot were used as the experimental materials,pathogen species were isolated and purified from the gerbera root rot by the tissue isolation method,then the pathogenicity of the isolates to gerbera plants were studied,finally the pathogen species were identified by using morphological and molecular systematics method based on rDNA-ITS and TEF-1α sequences,in order to make clear the fungal pathogen species of thegerbera root rot disease.The results showed that a total of 8 pathogenic strains of FZJf 1-1,FZJf 1-2,FZJf 1-3,FZJf 2-1,FZJf 2-2,FZJf 2-3,FZJf 3-1 and FZJf 3-2 were isolated from severely rot roots;pathogenicity test results showed that all the 8 strains were pathogenic to gerbera seedlings and could cause typical leaf wilt and root rot symptoms,among which strains FZJF 1-1 and FZJF 1-3 had the strongest pathogenicity,the plants developed disease after 3 weeks inoculation,followed by strain FZJF 2-1,the plants developed disease after 5 weeks inoculation,while the other strains were less pathogenicity;6 strains of FZJf 1-1,FZJf 1-2,FZJf 1-3,FZJf 2-1,FZJf 2-3 and FZJf 3-2 were identified as Fusarium oxysporum,one strain of FZJf 2-2 was identified as F.solani and one strain of FZJf 3-1identified as F.proliferatumby morphological and molecular phylogenetic characteristics.F.oxysporum species were the dominant pathogens,and F.solani and F.proliferatum were weak pathogens.

References:

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Last Update: 2021-11-13