|Table of Contents|

Generation of SlHSP17.7 Silencing Tomato and Analysis of Its Resistance to Low Temperature

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年15
Page:
1-6
Research Field:
Publishing date:

Info

Title:
Generation of SlHSP17.7 Silencing Tomato and Analysis of Its Resistance to Low Temperature
Author(s):
WU Yuanyuan1; 2; SHI Jiewei1; CHANG Chenliang1; LYU Shuwen2; JIANG Jing1
(1.College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110866;2.Vegetable Research Institute,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110866)
Keywords:
tomato; SlHSP17.7; cold stress; function analysis
PACS:
-
DOI:
10.11937/bfyy.20205427
Abstract:
Chmielewskii which can resist low-temperature was used as the experiment material,SlHSP17.7 silencing vector was successfully transformed into chmielewskii by Agrobacterium tumefaciens-mediated method.In order to determine the relationship between SlHSP17.7 and low temperature tolerance of tomato,the low temperature tolerance of chmielewskii silencing SlHSP17.7 was studied by measuring the relative conductivity,malondialdehyde content and NBT staining of reactive oxygen species.The results showed that the degree of membrane lipid peroxidation and the content of reactive oxygen species of SlHSP17.7 silencing lines were higher than the control ,and they were sensitive to low temperature.So,SlHSP17.7 plays an important role in improving the low temperature tolerance of tomato seedlings.The results provided a new idea for improving the low temperature tolerance of tomato seedlings and the cultivation of the resistant tomatoes.

References:

[1]NEELAM K S,YEON K K,ANIL G.Rice sHsp genes:Genomic organization and expression profiling under stress and development[J].BMC Genomics,2009（10）:393.[2]左丽萍,张瑞华,金桂秀,等.OsHsp18.0-CI调控水稻对白叶枯病的抗性[J].植物病理学报,2018（1）:1-13.[3]ZHAI M,SUN Y,JIA C,et al.Over expression of jrshsp17.3,gene from Juglans regia,confer the tolerance to abnormal temperature and nacl stresses[J].J Plant Biol,2016,59:549-558.[4]PARK S M,KIM K P,JOE M K,et al.Tobacco class I cytosolic small heat shock proteins are under transcriptional and translational regulations in expression and heterocomplex prevails under the high temperature stress condition in vitro[J].Plant Cell Environ,2015,38:767-776.[5]ZHOU Y L,CHEN H H,CHU P,et al.NnHSP17.5,a cytosolic class Ⅱ small heat shock protein gene from Nelum bo nucifera,contributes to seed germination vigor and seedling thermotolerance in transgenic Arabidopsis[J].Plant Cell Rep,2012,31:379-389.[6]CHAUHAN H,KHURANA N,NIJHAVAN A,et al.The wheat chloroplastic small heat shock protein (sHSP26) is involved in seed maturation and germination and imparts tolerance to heat stress[J].Plant Cell Environ,2012(35):1912-1931.[7]LU Z,WEI J H,YIKE G,et al.A cytosolic class Ⅱ small heat shock protein,PfHSP17.2,confers resistance to heat,cold,and salt stresses in transgenic Arabidopsis[J].Genetics and Molecular Biology,2018,41(3):649-660.[8]王丽,赵春梅,王义菊,等.过量表达叶绿体小分子热激蛋白提高番茄的抗寒性[J].植物生理与分子生物学学报,2005,31(2):167-174．[9]郭鹏,隋娜,于超,等.转入甜椒热激蛋白基因CaHSP18提高番茄的耐冷性[J].植物生理学通讯,2008,44(3):409-412.[10]GUO S J,ZHOU H Y,ZHANG X S,et al.Overexpression of CaHSP26 in transgenic tobacco alleviates photoinhibition of PSⅡ and PSⅠ during chilling stress under low irradiance[J].Plant Physiol,2007,164(2):126-136.[11]ZHANG N,SHI J W,ZHAO H Y,et al.Activation of small heat shock protein (SlHSP17.7) gene by cell wall invertase inhibitor (SlCIF1) gene involved in sugar metabolism in tomato[J].Gene,218,679:90-99.[12]陈双臣,刘爱荣,王凤华,等.农杆菌介导的番茄Micro-Tom遗传转化体系的建立[J].华北农学报,2010,25(2):112-115.[13]苏彩霞,李君明,霍秀文,等.番茄遗传转化体系的建立及LC和GFP标记基因的表达[J].中国农学通报,2007,23(6):131-136.[14]FRARY A,EARLE E D.An examination of factorsaffecting the efficiency of Agrobacterium-mediated transformation of tomato[J].Plant Cell Reports,1996（16）:235-240.[15]LING H Q,KRISELEIT D,GANAL M W．Effect of ticarcillin/potassium clavulanate on callus growth and shoot regeneration in Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum Mill．)[J].Plant Cell Rep,1998,17(11):843-847.[16]付超,王婷婷,银利辉,等.农杆菌介导番茄遗传转化的相关因素优化[J].分子植物育种,2013,11(5):592-599.[17]姜娜娜,赵传志,赵光敬,等.番茄果实特异性启动子的克隆与遗传转化研究[J].生物技术通报,2012(1):74-78.[18]SHARMA M K,SOLANKE A U,JANI D,et al.A simple and efficient Agrobacterium-mediated procedure for transformation of tomato[J].Journal of Biosciences,2009,34(3):423-433.[19]陈珍,朱诚.农杆菌介导的番茄遗传转化体系优化研究[J].浙江大学学报(农业与生命科学版),2008,34(6):615-620.[20]高佩,尹锐,林彦萍,等.农杆菌介导的番茄遗传转化研究进展[J].北方园艺,2016(14):192-197.[21]陈火英,张建华,钟建江,等.番茄下胚轴离体培养植株再生及其组织学观察[J].西北植物学报,2000,20(5):759-765.[22]丁杨林,施怡婷,杨淑华.植物响应低温胁迫的分子机制研究[J].生命科学,2015,27(3):398-405.[23]张瑶,王傲雪.植物响应低温胁迫的分子机制研究进展[J].黑龙江大学自然科学学报,2018,35(5):566-575.

Memo

Memo:
-
Last Update: 2021-11-18