|Table of Contents|

Genetic Characteristics of Rehmannia glutinosa Based on EST-SSR Marker

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年15
Page:
119-125
Research Field:
Publishing date:

Info

Title:
Genetic Characteristics of Rehmannia glutinosa Based on EST-SSR Marker
Author(s):
DONG Ruirui1; DONG Chengming1; 2; 3; SHEN Penglong1; YU Mengjuan1; WANG Xinyuan1; ZHANG Yanyan1
(1.School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou,Henan 450046;2.Dao-di Herbs Ecological Cultivation Engineering Research Center of Henan Province,Henan University of Chinese Medicine,Zhengzhou,Henan 450046;3.Provincial and Ministry Co-construction of Collaborative Innovation Center About Prevention and Treatment of Respiratory Diseases in Traditional Chinese Medicine,Henan University of Chinese Medicine,Zhengzhou,Henan 450046)
Keywords:
Rehmannia glutinosa; EST-SSR; variety; genetic characteristics
PACS:
-
DOI:
10.11937/bfyy.20204900
Abstract:
Taking the new Rehmannia glutinosa variety ‘Huaizhong No.1’ and the five mainstream varieties of genuine producing area ‘Jinjiu’‘Huaifeng’‘Beijing No.3’‘Qinhuai’‘85-5’,a total of 60 samples were taken as test materials,using EST-SSR molecular marker method,to study the genetic characteristics of different varieties of Rehmannia glutinosa,in order to provide reference for molecular marker assisted breeding.The results showed that 9 pairs of primers with polymorphism were selected from 68 pairs of primers.The number of alleles detected by each pair of primers were 8-25,with an average of 15 alleles.A total of 137 alleles (the range of molecular weight variation is 100-500 bp) were detected,and the percentage of polymorphic sitesis 97.08%.The distribution range of SSR polymorphic information content (PIC) was 0.850 1-0.926 4,with an average of 0.900 7.Effective alleles were 1.153 1-1.201 2,Nei′s gene diversity was 0.085 7-0.111 3,Shannon index was 0.124 6-0.160 5,and the genetic distance of different breeds ranges from 0.148 6 to 0.590 9.The cluster analysis results showed that when the coefficient of genetic similarity was 0.59,the 6 varieties were divided into 3 groups.At the genetic similarity coefficient of 0.79,10 samples of each variety were clustered into one group.It shows that SSR molecular markers can be used to identify the genetic relationship between different varieties of Rehmannia glutinosa and verify the purity of the varieties.

References:

[1]国家药典委员会.中华人民共和国药典（一部）[S].北京:中国医药科技社,2015.[2]付国辉,杜鑫.地黄化学成分及药理作用研究进展[J].中国医药科学,2015,5(15):39-41.[3]李建军,王莹,贾国伦,等.怀地黄不同主栽品种叶形态特征比较[J].中国中药杂志,2012,37(14):2061-2066.[4]徐守真.怀地黄不同品种间形态、生理、产量及品质的比较研究[D].新乡:河南师范大学,2013.[5]李建军,任美玲,王君,等.怀地黄新品种选育研究[J].中药材,2016,39(7):1452-1456.[6]安泽伟,赵彦宏,程汉,等.橡胶树EST-SSR标记的开发与应用[J].遗传,2009,31(3):311-319.[7]李明杰.基于地黄转录组的EST-SSR标记开发及块根发育机理研究[D].郑州:河南农业大学,2013.[8]李重,胡伟明,杨天顺,等.用于枸杞品种鉴定的多重EST-SSR标记的建立[J].分子植物育种,2017,15(10):4066-4070.[9]李军,董彬,张超,等.桂花EST-SSR引物开发及在品种鉴定中的应用[J].浙江农林大学学报,2018,35(2):306-313.[10]张晗,姚凤霞,刘永杰,等.黄淮海地区主要冬小麦品种的EST-SSR遗传多样性分析[J].分子植物育种,2010,8(2):297-302.[11]郭冠瑛.地黄大容量转录组文库的构建及EST-SSR标记的开发与鉴定[D].郑州:河南农业大学,2013.[12]庞丁玮,武素然,张军,等.基于转录组测序的黑枣EST-SSR引物开发与鉴定分析[J].分子植物育种,2021,19(1):200-208.[13]周立,罗聪,何堂熹,等.基于EST-SSR标记的芒果品种遗传多样性与亲缘关系分析[J].分子植物育种,2020,18(11):3797-3806.[14]王型力,骆甲,姚张秀,等.基于EST-SSR标记的部分玉兰属植物亲缘关系分析[J].分子植物育种,2018,16(24):8087-8095.[15]周延清,景建洲,李振勇,等.利用RAPD和ISSR分子标记分析地黄种质遗传多样性[J].遗传,2004(6):922-928.[16]周春娥,谷凤平,周延清.基于SRAP分析怀地黄种质的遗传多样性[J].贵州农业科学,2012,40(2):4-7.[17]王丰青,田云鹤,黄勇,等.18个地黄种质的表型性状及相关统计学分析[J].植物资源与环境学报,2015,24(1):28-35.[18]赵正伟,刘兢文,孙冠庆.怀地黄品种金九的形态特征及高产栽培技术[J].安徽农学通报,2019,25(20):42-43.[19]LI X J,JIANG C,XU N,et.al.Sorting and identification of Rehmannia glutinosa germplasm resources based on EST-SSR,scanning electron microscopy micromorphology,and quantitative taxonomy[J].Industrial Crops & Products,2018,123:303-314.[20]张爱玲,马月辉,陈秀梅,等.不等微卫星座位数目对Nei氏标准遗传距离估测精度的影响[J].畜牧兽医学报,2005(5):431-433.

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Last Update: 2021-11-23