|Table of Contents|

Isolation and Identification of Strawberry Leaf Spot Disease in Shangqiu

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年17
Page:
38-43
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Strawberry Leaf Spot Disease in Shangqiu
Author(s):
ZHU Xiaoqin; ZHANG Qi; ZHENG Aizhen; HAN Shuang; PEI Dongli
(Henan Provincial Key University Laboratory for Plant-microbe Interactions，Shangqiu Normal University，Shangqiu,Henan 476000)
Keywords:
strawberry; leaf spot disease; morphological character; molecular identification
PACS:
-
DOI:
10.11937/bfyy.20204762
Abstract:
Taking the leaves of strawberry in Shuangba town,Shangqiu as material,the pathogen of strawberry leaf spot disease by tissue isolation was investigated，in order to provide a reference for the control of strawberry leaf spot disease.The results showed that a fungus SQZQ-06 from strawberry leaf spot in Shuangba town,Shangqiu,was identified as Pestalotiopsis maculans.The pathogenicity of the strain was determined by Coch rule,and it was found that the strain could lead to the necrosis of the strawberry leaves.The 18S rRNA gene of SQZQ-06 was amplified by PCR for molecular identification,and the length was 589 bp.Blast result in NCBI and phylogenetic tree analysis suggested that SQZQ-06 was Pestalotiopsis maculans.According to the morphological characteristics and molecular identification of the fungus,SQZQ-06 was identified as Pestalotiopsis maculans.

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Last Update: 2021-12-09