|Table of Contents|

Identification of the Pathogen of Capsicum Anthracnose and Screening of Synergistic Agents in Guizhou Province

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年14
Page:
21-30
Research Field:
Publishing date:

Info

Title:
Identification of the Pathogen of Capsicum Anthracnose and Screening of Synergistic Agents in Guizhou Province
Author(s):
LUO Yue1; 2; 3; LIU Xudong1; 2; 3; HAN Lei1; 2; 3; YAO Xiaolong1; 2; 3; GENG Guangdong3; WU Xiaomao1; 2; 3
(1.Institute of Crop Protection,Guizhou University,Guiyang,Guizhou 550025;2.The Provincial Key Laboratory for Agricultural Pest Management in Mountainous Region,Guiyang,Guizhou 550025；3.Agricultural College,Guizhou University,Guiyang,Guizhou 550025)
Keywords:
pepper; anthracnose; identification; control agent; synergy
PACS:
-
DOI:
10.11937/bfyy.20204677
Abstract:
Capsicum anthracnose samples collected from Guiyang and Zunyi in Guizhou Province were used to isolate the pathogen by tissue isolation method.The pathogenicity of the pathogen was determined according to Koch′s law.The pathogen causing pepper anthracnose in Guizhou Province was studied according to the morphological characteristics and rDNA-ITS sequence analysis;mycelial growth inhibition method was used to study the sensitivity of pathogenic strains to 11 original drugs such as thiophanate methyl and 6 finished products such as 10% difenoconazole.On this basis,different combinations of drugs with different mechanisms were selected to determine the inhibitory activity.The results showed that,combining morphology and molecular biology,it was determined that it was the pathogen of capsicum anthracnose,which were C.acutatum,C.scovillei and suspected new species (Colletotrichum sp.).The sensitivity test results showed that the selected funguscides had a certain degree of inhibitory effect on the strains.The 4 original medicines,including prochloraz,dibenzamide,pyraclostrobin,and tebuconazole,had relatively stable efficacy against 3 strains of capsicum anthracnose.The inhibitory effect was better,capsicum anthracnose was more sensitive to 2 funguscides,22.5% picoxystrobin and 10% difenoconazole,with EC50 values of 1.60 mg?L-1 and 2.12 mg?L-1,respectively.When alcohol and pyraclostrobin were compounded at 3∶1 and 1∶1,the SR was 1.745 3 and 2.061 1,respectively,and the synergistic effect was the best at 1∶1.When picoxystrobin and prochloraz were compounded at 1∶3,1∶1 and 1∶5,the SR was 1.723 4,1.661 1 and 1.658 5 respectively,and the synergistic effect of 1∶3 was the best.In conclusion,the pathogens causing capsicum anthracnose in Guizhou Province are GY-1 (C.acutatum);ZY-1 (C.scovillei);ZY-2 suspected new species (Colletotrichum sp.).

References:

[1]KIM J S,AN C G,PARK J S,et al.Carotenoid profiling from 27 types of paprika(Capsicum annuum L.) with different colors,shapes,and cultivation methods[J].Food Chemistry,2016,201:64-71.[2]ZHANG X M,ZHANG Z H,XIAO-ZHEN G U,et al.Genetic diversity of pepper (Capsicum spp.) germplasm resources in China reflects selection for cultivar types and spatial distribution[J].Journal of Integrative Agriculture,2016,15(9):1991-2001.[3]牟玉梅,毛妃凤,张绍刚.贵州省辣椒产业现状与发展建议[J].中国蔬菜,2020(2):10-12.[4]SOUZA L C S,ASSIS L A G,CATARINO A D,et al.Screening of chilli pepper genotypes against anthracnose(Colletotrichum brevisporum)[J].Emirates Journal of Food and Agriculture,2019,31:919-929.[5]SHIN J H,HAN J H,PARK H H,et al.Optimization of polyethylene Glycol-Mediated transformation of the pepper anthracnose pathogen Colletotrichum scovillei to develop an applied genomics approach[J].The Plant Pathology Journal,2019,35(6):575-584.[6]KHALIMI K,DARMADI A A K,SUPRAPTA D N.First report on the prevalence of Colletotrichum scovillei associated with anthracnose on chili pepper in Bali,Indonesia[J].International Journal of Agriculture and Biology,2019,22(2):363-368.[7]MISHRA R,ROUT E,MOHANTY J N,et al.Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)[J].Biotech,2019,9(1):9.[8]PRAPASRI S,KONGYUTH L,RAPEEWAN S,et al.Bacillus sp.strain M10 as a potential biocontrol agent protecting chili pepper and tomato fruits from anthracnose disease caused by Colletotrichum capsici[J].Biocontrol,2018,63(6):833-842.[9]易兰.辣椒炭疽病病原菌的分离鉴定和辣椒抗炭疽病材料的筛选[D].雅安：四川农业大学,2019.[10]高福喜.辣椒炭疽病的发病规律及综合防治措施[J].农技服务,2014,31(7):127.[11]PEDROSO C,DIAS-NETO J J,ROSSATO M,et al.Managing sweet pepper anthracnose by choice of planting season,organic soil mulch,fungicide and moderate nitrogen fertilization[J].Phytoparasitica,2020,48(4):525-533.[12]REN L,WANG S F,SHI X J,et al.Characterisation of sensitivity of Colletotrichum gloeosporioides and Colletotrichum capsici,causing pepper anthracnose,to picoxystrobin[J].Journal of Plant Diseases and Protection,2020,127(5):657-666.[13]GAO Y,LI X,HE L,et al.Effect of application rate and timing on residual efficacy of pyraclostrobin in the control of pepper anthracnose[J].Plant Disease,2019,104(3):958-966.[14]毕慧强.怎样防治辣椒炭疽病[J].新农业,1987(Z1):78.[15]张新强,桑维钧,倪云跃,等.中草药提取物对辣椒炭疽病菌和番茄灰霉病原菌的抑制效果[J].贵州农业科学,2012,40(4):100-102.[16]任艳玲,王涛,王力,等.12种杀菌剂对辣椒炭疽病菌的毒力测定[J].中国瓜菜,2019,32(7):31-33.[17]程欢欢,余水,姚伟伟,等.辣椒炭疽病生防芽孢杆菌的筛选及田间防效[J].河南农业大学学报,2019,53(4):568-573.[18]李小霞,肖仲久.贵州省辣椒炭疽病病原菌鉴定及室内毒力测定[J].广东农业科学,2011,38(17):55-57,238.[19]王妮,尹显慧,彭丽娟,等.辣椒炭疽病病原鉴定及其杀菌剂毒力测定[J].植物保护,2019,45(4):216-223.[20]张中义.植物病原真菌学[M].成都：四川科学技术出版社,1988.[21]高杨杨,禾丽菲,李北兴,等.山东省辣椒炭疽病病原菌的鉴定及高效防治药剂的筛选[J].中国农业科学,2017,50(8):1452-1464.[22]DAMM U,CANNON P F,WOUDENBERG J H C,et al.The C.acutatum species complex[J].Study in Mycology,2012,73(1):37-113.[23]KANTO T,UEMATSU S,TSUKAMOTO T,et al.Anthracnose of sweet pepper caused by C.scovillei in Japan[J].J Gen Plant Pathol,2014,80:73-78.[24]刘方玲.四川辣椒炭疽病的病原学研究[D].雅安：四川农业大学,2015.[25]任璐,周建波,刘慧平,等.辣椒炭疽病菌对啶氧菌酯的敏感基线及抗性突变体生物学性状[J].植物保护,2017,43(6):29-37.[26]石玉星,曹俊宇,任璐,等.辣椒炭疽病菌抗啶氧菌酯突变体渗透压敏感性及交互抗性[J].山西农业科学,2017,45(6):1002-1005.

Memo

Memo:
-
Last Update: 2021-10-27