|Table of Contents|

Identification and Biological Characteristics of Wild Lentinula edodes From Papua New Guinea

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年14
Page:
143-152
Research Field:
Publishing date:

Info

Title:
Identification and Biological Characteristics of Wild Lentinula edodes From Papua New Guinea
Author(s):
LIAO Zhen1; 2; LIN Zhanxi1; 2; XU Zhiwen1; 2; LI Qiaoqi1; 2; WANG Lifang1; 2; XUE Zhixiang1; 2
(1.College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002;2.National Engineering Research Center of JUNCAO,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002)
Keywords:
Lentinula lateritia; nuclear gene sequence; biological characteristics; orthogonal test
PACS:
-
DOI:
10.11937/bfyy.20204661
Abstract:
A wild Lentinula edodes from Papua New Guinea was used as test material,we used sequence analysis of ITS,LSU and SSU for molecular identification,and used single factor tests and orthogonal tests to explore its mycelial biological characteristics.In order to clarify the taxonomic status of the wild Lentinula edodes and provide a reference for the artificial cultivation and further research of Lentinula lateritia.The results showed that the length of the ITS sequence was 725 bp,the length of LSU sequence was 934 bp,and the length of SSU sequence was 1 100 bp,which had the highest similarity with Lentinula lateritia.The strain was identified as Lentinula lateritia.The results of biological characteristics showed that the optimal carbon source for the growth of wild Lentinula edodes mycelial was fruticose,the optimal nitrogen source was beef extract,the optimal pH was 4.0,and the optimal growth temperature was 25 ℃.

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Last Update: 2021-11-13