|Table of Contents|

Establishment of Rapid Propagation Technology for Impatiens rubrostriata in vitro

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年15
Page:
57-64
Research Field:
Publishing date:

Info

Title:
Establishment of Rapid Propagation Technology for Impatiens rubrostriata in vitro
Author(s):
WEN Yonghui1; CHEN Shuangxiu2; WANG Qiong1; AI Xingmei1; HUANG Meijuan1; HUANG Haiquan1
(1.College of Gardening and Horticulture,Southwest Forestry University，Yunnan Province Engineering Research Center for Functional Flower Resources and Industrialization，Southwest Research Center for Landscape Architecture Engineering，State Forestry and Grassland Administration，Garden and Horticulture Flower R&D Center of Southwest Forestry University，Kunming，Yunnan 650224;2.Forest Station of Hekou Town，Xundian County，Xundian，Yunnan 655201)
Keywords:
Impatiens rubrostriata; primary culture; proliferation culture; rooting culture; sterilization method; cefotaxime sodium
PACS:
-
DOI:
10.11937/bfyy.20204302
Abstract:
The budding stems of Impatiens rubrostriata were used as materials.The explants were treated with different sterilants，and primary culture，proliferation and rooting culture.They were performed on the medium with different types of hormoneand concentrations to screen the best sterilization method，induction，proliferation and rooting medium of Impatiens rubrostriata.The results showed that the optimal sterilization method for Impatiens rubrostriatais 75% alcohol，10 seconds+0.1% mercury chloride for 6 minutes，the survival rate was 100%.When 20 mg·L-1cefotaxime sodium was added to the primary medium，the bacterial effect was better，the survival rate was 100%.The most suitable primary medium was MS+1.0 mg·L-1 6-BA+0.3 mg·L-1 NAA，under this circumstances，the seedlings were strong and the growth was fast.The optimal proliferation medium was MS+1.0 mg·L-1 6-BA+0.3 mg·L-1NAA+0.010 mg·L-1TDZ，the proliferation coefficient was 6.65，the seedlings grew fast.The most suitable rooting medium was MS+0.10 mg·L-1 IBA，and the rooting rate was 100%.The in vitro rapid propagation system for Impatiens rubrostriata was established，which will lay a good technical foundation for development and utilization of Impatiens rubrostriata.

References:

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Last Update: 2021-11-22