|Table of Contents|

Optimization of Agaricus bisporus Liquid Inoculum Cultivation by Response Surface Methodology and Preparation of Reduced Liquid Inoculum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年14
Page:
134-142
Research Field:
Publishing date:

Info

Title:
Optimization of Agaricus bisporus Liquid Inoculum Cultivation by Response Surface Methodology and Preparation of Reduced Liquid Inoculum
Author(s):
LI Yanan; XU Zhixu; SHANG Di; ZHANG Yan; QIU Liyou; GAO Yuqian
(College of Sciences,Henan Agricultural University/Key Laboratory of Enzyme Engineering of Agricultural Microbiology,Ministry of Agriculture,Zhengzhou,Henan 450002)
Keywords:
Agaricus bisporus; compost extracts; calcium peroxide; response surface methodology; reduced liquid inoculum
PACS:
-
DOI:
10.11937/bfyy.20204199
Abstract:
Taking Agaricus bisporus strain As2796 as the test material,the optimal cultivating conditions for Agaricus bisporus liquid inoculum in shake flasks were investigated by single factor experiment combined with response surface method,in order to provid a reference for the efficient preparation of Agaricus bisporus liquid incoulum and reduced liquid incoulum.The results showed that addition of the compost water extract 50% and calcium peroxide 0.010 0 g·(100mL)-1 in the enriched medium and the liquid volume 80 mL in the 250 mL triangular bottle was the optimal cultivating conditions,the biomass of the mushroom liquid spawn reached 0.95 g·(100mL)-1,which was 57.3% higher than that of enriched medium.The liquid spawn was concentrated by suction filtration and solidificated with glue Y genarating reduced liquid inoculum.After stored at 4 °C for 30 days,the growth rate of the reduced liquid inoculum was similar with the liquid spawn before solidification.

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Last Update: 2021-11-13