|Table of Contents|

Screening and Identification of Potassium Solubilizing Bacteria in Soil Composted with Chinese Medicine Residue

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年09
Page:
87-92
Research Field:
Publishing date:

Info

Title:
Screening and Identification of Potassium Solubilizing Bacteria in Soil Composted with Chinese Medicine Residue
Author(s):
HU Shan; LIANG Weiqu; HUANG Hao; XU Cong; LUO Huajian; CHEN Shili
(Dongguan Agricultural Science Research Institution,Dongguan,Guangdong 523000)
Keywords:
potassium bacteria; Cellulosimicrobium cellulans; potassium-dissolving
PACS:
-
DOI:
10.11937/bfyy.20203346
Abstract:
Chinese medicine residue with soil after two months composting was used as test material.The isolation,activity evaluation and identification of postassium bacteria were studied by using dilution plate method,in order to provide a reference for the precparation of efficient bioorganic fertilizer reserve strains.The results showed that six strains (DGNK-JJ1,DGNK-JJ2,DGNK-JJ3,DGNK-JJ4,DGNK-JJ5 and DGNK-JJ6） were isolated.After five days fermentation under the condition of 30 ℃,150 r?min-1,the content of available potassium in fermentation liquid of DGNK-JJ1 reached a maximum of 1.84 mg?L-1.It was 93.72% higher than the blank control.Together with colony morphology,physiological and biochemical characteristics,16S rDNA sequence analysis revealed DGNK-JJ1 as Cellulosimicrobium cellulans.K-B disk diffusion method was used to test the drug sensitivity of DGNK-JJ1.The strain was highly sensitive to chloramphenicol,doxycycline,vancomycin,enrofloxacin,rifampicin,gentamicin.Strain DGNK-JJ1 can be used as a reserve strain for the development of biological potassium fertilizer.

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Last Update: 2021-08-06