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Co-culture System of Bletilla striata Callus With Endophytic Fungi and Changing Contents of Secondary Metabolites

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年14
Page:
128-134
Research Field:
Publishing date:

Info

Title:
Co-culture System of Bletilla striata Callus With Endophytic Fungi and Changing Contents of Secondary Metabolites
Author(s):
LI Lin1; SHANGGUAN Yanni1; HUANG Ceyin1; WU Mingkai2; XU Lingling3; XU Delin1
(1.School of Basic Medicine Sciences,Zunyi Medical University,Zunyi,Guizhou 563000;2.Institute of Modern Chinese Herbal of Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006;3.School of Biological and Environmental Engineering,Xi′an University/Key Laboratory of Plant-microbe Collaboration Research,Xi′an,Shaanxi 710065)
Keywords:
Bletilla striata; callus; endophytic fungi; secondary metabolites; co-culture
PACS:
-
DOI:
10.11937/bfyy.20203320
Abstract:
The callus of Bletilla striata were used as material,co-cultured with endophytic fungi method was used to study the growth rate of callus with endophyte,and contents changing of four main secondary metabolites in the period,in order to provide a reference for the development and utilization of endophytes in Bletilla striata.The results showed that the areas of 7 fungal strains co-cultured with callus of Bletilla striata for 3 days and 6 days were higher than that of endophyte single culture,and the callus proliferation rate of callus of each treatment group was significantly lower than that of control (CK) group for 9 days.And one of the tested endophytic fungi grew slowly,and displayed almost the same fungi area in co-culture treatment with culture alone for 3,6,9 days.Furthermore,the secondary metabolism contents of callus in Bletilla striata co-cultured with 8 endophytic fungi,showed that different endophytic fungi had various effects on the accumulation trend of dactylorhin A,4-Hydroxybenzaldehyde,militarine,and coelonin.

References:

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Last Update: 2021-11-13