|Table of Contents|

Molecular Identification and Phylogenetic Analysis of Three Wild Edible Mushrooms in Qinhuangdao Area

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年08
Page:
111-116
Research Field:
Publishing date:

Info

Title:
Molecular Identification and Phylogenetic Analysis of Three Wild Edible Mushrooms in Qinhuangdao Area
Author(s):
GENG Xiaojin1; 2; JIANG Qiumeng1; ZHANG Tengyang1; FU Yajuan1; 2; KONG Hong1; 2
(1.College of Life Science,Langfang Normal University,Langfang,Hebei 065000;2.Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province/Edible and Medicinal Fungi Research and Development Center of Hebei Universities,Langfang,Hebei 065000)
Keywords:
Qinhuangdao area; edible mushroom; ITS; molecular identification; phylogenetic analysis
PACS:
-
DOI:
10.11937/bfyy.20203174
Abstract:
In order to lay a foundation for the rational development and artificial domestication of wild rou mushroom,ga mushroom and song mushroom in Qinhuangdao area,the ITS regions of rDNA genes were amplified using ITS1 and TIS4 as primers,cloned and sequenced.ITS sequences were analyzed via NCBI blast program,meanwhile phylogenetic tree were constructed,respectively，in order to provide a foundation for the rational development and artificial domestication of the three wild edible mushrooms.The results showed that rou mushroom had 99% similarity with Chroogompus rutilus (MG4578511),the similarity between ga mushroom and Lactarius akahatsu (EF6850451) was up to 100%,but song mushroom merely had 96% similarity with Suillus granulatus(MF4211071).Phylogenetic analysis showed that there was a close genetic relationship between rou mushroom and C.rutilus,their genetic distance reached to 0.Ga mushroom and L.akahatsu also had higher relationship,the genetic distance between them was 0.Song mushroom had a lower genetic relationship to Suillus granulatus (0033).Therefore,rou mushroom and ga mushroom in Qinhuangdao area were at the molecular level identified as C.rutilus and L.akahatsu,respectively.Song mushroom,which was another kind of wild edible fungi in Qinhuangdao area,belonged to Suillus.

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Last Update: 2021-08-06