|Table of Contents|

Determination of Pollen Vitality and in vitro Germination of Pollen in Forsythia suspensa

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年10
Page:
112-117
Research Field:
Publishing date:

Info

Title:
Determination of Pollen Vitality and in vitro Germination of Pollen in Forsythia suspensa
Author(s):
YANG Yanhong; SONG Guiquan; DONG Hui; DUAN Genhua; LI Guangyao; JIANG Qianqian
(College of Biology and Agricultural Engineering，Weifang University/Key Laboratory of Biochemistry & Molecular Biology in Universities of Shandong，Weifang，Shandong 261061)
Keywords:
Forsythia suspensa; medium components; in vitro germination; pollen vitality
PACS:
-
DOI:
10.11937/bfyy.20202766
Abstract:
Pollen of Forsythia suspensa was used as the experimental material，the effects of sucrose，CaCl2，H3BO3,PEG and different culture times on pollen germination and pollen tube growth of Forsythia suspensa were studied by using pollen liquid culture.By comparing in vitro pollen germination culture method，TTC staining method and acetic acid magenta staining method，the suitable method for rapid determination of pollen vitality of forsythia was obtained,in order to provide a reference for cross breeding of Forsythia suspensa.The results showed that,the concentrations of，sucrose CaCl2，H3BO3 and PEG in the medium had significant influence on pollen germination and pollen tube growth,the optimal medium combination for in vitro germination of forsythia pollen was 15% sucrose+0.015% H3BO3+0.020% CaCl2+10% PEG,the optimal culture time was 3 hours，the higher pollen germination rate and longest pollen tube reached 87.20% and 181.20 μm respectively.Acetic acid magenta staining method can be used for the rapid determination of pollen vitality of forsythia，resembling the result of in vitro germination.

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Last Update: 2021-08-19