|Table of Contents|

Correlation Analysis of Nutrient Components and Enzyme Activity of Cordyceps militaris Cultivated in Different Substrates

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年08
Page:
99-105
Research Field:
Publishing date:

Info

Title:
Correlation Analysis of Nutrient Components and Enzyme Activity of Cordyceps militaris Cultivated in Different Substrates
Author(s):
ZHAO Ying; MA Xiaoying; CHEN Xun; XIAO Jun
(Edible Fungus Institute,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161)
Keywords:
Cordyceps militaris; intracellular enzyme; nutritional components; correlation analysis
PACS:
-
DOI:
10.11937/bfyy.20202527
Abstract:
Cordyceps militaris fruiting body was used to compare the polysaccharide and protein content of Cordyceps militaris fruiting body in wheat,rice and silkworm chrysalis as the medium,and the enzyme activity indexes such as laccase,cellulase,amylase,protease,filter paper enzyme and polyphenol oxidase,etc,so as to analyze the correlation between intracellular enzyme activity and polysaccharide and protein content,in order to provide reference for the cultivation of Cordyceps militaris fruiting body with high protein and polysaccharide.The results showed that polysaccharide and protein content in Cordyceps militaris were all higher than those in wheat and rice matrix.Also,as the double test analysis showed,the accumulation of polysaccharides in Cordyceps militaris fruiting body was significantly positively correlated with cellulase activity,and negatively correlated with protease activity.The accumulation of protein in Cordyceps militaris fruiting body was significantly positively correlated with amylase activity and negatively correlated with polyphenol oxidase activity.

References:

[1]左克源，裘军，李维亮，等.人工蛹虫草子实体对小鼠免疫功能的影响[J].医药导报,2007(3):227-229.[2]XIA Y L,LUO F F,SHANG Y F,et al.Fungal cordycepin biosynthesis is coupled with the production of the safeguard molecule pentostatin[J].Cell Chemical Biology,2017,24:1479-1489.[3]MAMTA S,MEHROTRA V,AMITABH P,et al.Phytochemical and antimicrobial activities of Himalayan Cordyceps sinensis (Berk.) Sacc[J].Indian Journal of Experimental Biology,2015,53(1):36-43.[4]RUMA I M,PUTRANTO E W,KONDO E,et al.Extract of Cordyceps militaris inhibits angiogenesis and suppresses tumor growth of human malignant melanoma cells[J].International Journal of Oncology,2014,45(1):209-218.[5]YANG H P,HSIN L Y,LING E Y,et al.Cordycepin and a preparation from Cordyceps militaris inhibit malignant transformation and pro-liferation by decreasing EGFR and IL-17RA signaling in a murineoral cancer model[J].Murine Oncotarget,2017,8(55):93712-93728.[6]WONG J H,NG T B,WANG H,et al.Cordymin,an antifungal peptide from the medicinal fungus Cordyceps militaris[J].Phytomedicine,2011,18(5):387-392.[7]林群英,吴亮亮,张锋伦,等.蛹虫草栽培过程中胞外蛋白酶活性变化研究[J].北方园艺,2014(17):148-150.[8]李娟,倪新江,樊守金,等.金顶侧耳不同生长期几种胞外酶活性变化[J].吉林农业大学学报，2006,28(6):619-622.[9]肖军,夏爽,陈珣,等.野生蛹虫草的分离及出草分子检测研究[J].北方园艺,2018(22):69-173.[10]李树英,李亚杰.利用柞蚕活蛹生产蛹虫草技术[J].中国蚕业2013,34(2):80-84.[11]张刚,汪天虹,张臻峰,等.产低温淀粉酶的海洋真菌筛选及研究[J].海洋科学,2002(2):3-5.[12]沈玉洁,张明春,向苇,等.高产酸性蛋白酶菌株的筛选及发酵条件研究[J].食品与发酵科技,2010,46(6):33-35.[13]孙晓燕,蔡昌利,徐丽莉,等.多糖含量测定方法的比较[J].现代中药研究与实践,2015(3):58-62.[14]李万芳,刘敬民,石俊英.不同产地北虫草蛋白质提取工艺及含量测定研究[J].辽宁中医杂志,2015,42(10):1944-1947.[15]刘雁英,李林辉,谢富刚,等.食用菌子实体分化发育研究进展[J].菌物研究,2010,8(1):57-62.[16]倪新江,丁立孝,潘迎捷,等.姬松茸在两种培养基上生长期间九种胞外酶活性变化[J].菌物系统,2001,20(2):222-227.[17]王孟兰,赵妍,陈明杰,等.食用菌半纤维素酶系研究进展[J].生物学杂志,2014,31(1):64-67.[18]韩增华,张丕奇,孔祥辉,等.黑木耳胞外酶活变化与栽培性状比较的研究[J].食用菌学报,2007,14(4):41-46.[19]宋爱荣,郭立忠,刘作亭,等.七个白色金针菇菌株发酵液中四种胞外酶活性的测定与分析[J].中国食用菌,1999,18(4):31-34.[20]宋吉玲,袁卫东,王伟科,等.桑黄菌液体培养过程中酶活及多糖含量变化规律[J].菌物学报,2019,38(11):1-10.

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Last Update: 2021-08-06