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Title:: Screening and Identification of Antagonistic Bacillus spp.Strains Against Snap Bean Root Rot Caused by Fusarium solani f.sp.phaseoli in Northeast China

Author(s):: WANG Jie1; LIU Huancheng1; TIAN Tian1; LIU Dajun2; FENG Guojun2; (1.Center of Biological,Harbin Academy of Agricultural Sciences,Harbin,Heilongjiang 150029；2.College of Modern Agriculture and Ecological Environment,Heilongjiang University,Harbin,Heilongjiang 150080)

Keywords:: biological control; snap bean; Fusarium solani f.sp.phaseoli; Bacillus velezensis

PACS:: -

DOI:: 10.11937/bfyy.20202522

Abstract:: Fusarium solani f.sp.phaseoli was taken as target bacteria,the Bacillus strains with strong antagonistic ability were screened from the rhizosphere (surrounding) soil of the diseased snap bean by the method of plate confrontation culture and control effect in greenhouse,in order to obtain the biocontrol resources of Fusarium root rot.The results showed that a strain of antagonistic Bacillus WJ-126 was screened and identified as Bacillus velezensis.Under the condition of artificial inoculation in greenhouse,the relative control effect of this strain on Fusarium root rot of snap bean was 60.92%.

References:

[1]ABEYSINGHE S.Biological control of Fusarium solani f.sp.phaseoli the causal agent of root rot of bean using Bacillus subtilis CA32 and Trichoderma harzianum RU01[J].Ruhuna Journal of Science,2007（2）:82-88.[2]EKE P,CHATUE G,WAKAM L N,et al.Mycorrhiza consortia suppress the fusarium root rot (Fusarium solani f.sp. phaseoli) in common bean (Phaseolus vulgaris L.)[J].Biological Control,2016,103:240-250.[3]TOGHUEO R M K,EKE P,GONZLEZ Z,et al.Biocontrol and growth enhancement potential of two endophytic Trichoderma spp.from Terminalia catappa against the causative agent of common bean root rot (Fusarium solani)[J].Biological Control,2016,96:8-20.[4]方中达.植病研究方法[M].北京:中国农业出版社,1998.[5]全鑫,薛保国,杨丽荣,等.生防菌株YB-81的鉴定及其对番茄灰霉病的防效[J].植物保护,2010,36(5):57-60.[6]卢庭婷,李涛,梁智群.水稻稻瘟病拮抗链霉菌的诱变选育[J].西南农业学报,2007,20(4):577-580.[7]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.[8]YAMAMOTO S,HARAYAMA S.PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains[J].Applied and Environmental Microbiology,1995,61(3):1104-1109.[9]HOLT J G,KRIEG N R,SNEATH P H A,et al.Bergey′s manual of determinative bacteriology[M].9th ed.Baltimore:Williams & Wilkins,1994.[10]王伟,李术娜,李红亚,等.大丽轮枝菌拮抗细菌菌株12-51的筛选鉴定与抗菌物性质分析[J].中国农学通报,2009,25(19):14-19.[11]杜淑涛,李术娜,朱宝成.白菜黑斑病拮抗细菌Bacillus velezensis DL-59的筛选鉴定及田间防效实验[J].河北农业大学学报,2010(6):51-56.[12]王伟,李术娜,李红亚,等.番茄灰霉病拮抗细菌的筛选与X-75菌株鉴定[J].园艺学报,2010,37(2):307-312.[13]高振峰.内生细菌ZSY-1对番茄灰霉病和早疫病的防治及促生效果研究[D].太谷:山西农业大学,2018.[14]赵昱榕,李磊,谢学文,等.贝莱斯芽胞杆菌ZF2对多主棒孢病菌防治效果[J].中国生物防治学报,2019,35(2):217-225.[15]康星星.贝莱斯芽孢杆菌CC09防治小麦全蚀病菌侵染的机制[D].南京:南京大学,2019.[16]杨可,郑柯斌,黄晓慧,等.海洋生境贝莱斯芽孢杆菌TCS001的鉴定及抑真菌活性[J].农药学学报,2018,20(3):333-339.[17]李生樟,陈颖,杨瑞环,等.一株拮抗黄单胞菌的贝莱斯芽孢杆菌的分离和鉴定[J].微生物学报,2019,59(10):1969-1983.[18]MATES A D P K,PONTES N D C,HALFELD-VIEIRA B D A.Bacillus velezensis GF267 as a multi-site antagonist for the control of tomato bacterial spot[J].Biological Control,2019,137:104013.[19]CHOEGYEONGJA K,JIN C.Bacillus velezensis S3-5 strain and method for the biological[J].Republic of Korea,2008,10:108-149.[20]PALAZZINI J M,DUNLAP C A,BOWMAN M J,et al.Bacillus velezensis RC218 as a biocontrol agent to reduce Fusarium head blight and deoxynivalenol accumulation:Genome sequencing and secondary metabolite cluster profiles[J].Microbiological Research,2016,192:30-36.[21]FERNAMDEZ MARTINEZ A I,VILLAVERDE FERNANDEZ M J,CASANOVA ROCA J A,et al.A pure culture of strain AH2 of the Bacillus velezensis species and a product for the biological control of phytopathogenic fungi,WO,ES2008/000109[P].2008-02-27.[22]KANJANAMANEESATHIAN M,WIWATTANAPATAPEE R,ROTNIAM W,et al.Application of a suspension concentrate formulation of Bacillus velezensis to control root rot of hydroponically-grown vegetables[J].New Zealand Plant Protection,2013,6(66):229-234.

Screening and Identification of Antagonistic Bacillus spp.Strains Against Snap Bean Root Rot Caused by Fusarium solani f.sp.phaseoli in Northeast China

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

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