|Table of Contents|

Genetic Diversity Analysis of Pea Germplasm Resources

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年02
Page:
1-9
Research Field:
Publishing date:

Info

Title:
Genetic Diversity Analysis of Pea Germplasm Resources
Author(s):
ZHANG Jiaxin; LI Jingyan; ZHU Shaowen; SHENG Yunyan; JI Peng
(College of Horticulture and Landscape Architecture，Heilongjiang Bayi Agricultural Reclamation University，Daqing，Heilongjiang 163319)
Keywords:
peas; phenotypic traits; SSR; seed resources; genetic diversity
PACS:
-
DOI:
10.11937/bfyy.20202088
Abstract:
Taking 34 pea germplasm resources as test materials,19 phenotypic traits were used to study the variability,diversity and principal component analysis,and the genetic diversity analysis was carried out in combination with SSR molecular markers in order to accelerate the research process of pea seed resources.The results showed that there were significant differences among the 19 traits among different materials,the average coefficient of variation was 45.05%,and the average genetic diversity index was 0.95.The first 7 components accounted for 74.807% of the expression information,among which the first principal component accounted for 18.350% of the expression information,and the first principal component presented a higher load in umbilical color,grain color,leaf area,stem diameter and color.Phenotypic trait analysis divided 34 materials into eight categories.Molecular marker study results showed that a total of 25 pairs of SSR primers with stable polymorphism were screened out from 118 pairs,115 difference sites were detected,with an average of 4.6 for each pair of primers,the effective allelic variation was 1.694 4,the proportion of effective allelic variation was 40.17%,and Shannon’s diversity information index was 0.594 1.By using NTSYS software progressive clustering analysis,the genetic similarity coefficient (GS) of 34 materials was 0.461-0.860,with an average of 0.661.The 34 materials introduced by SSR labeling analysis were also divided into eight categories.The correlation between the results of the two classification methods was (R=0.901 3,P=0.101 1>0.050) with a significant positive correlation.This study used SSR molecular markers to reveal the genetic background and relationship of pea germplasm resources at the molecular level,providing an important basis for the utilization of pea resources in the future.

References:

[1]吴星波.豌豆核心种质资源遗传多样性研究[D].重庆：西南大学,2014.[2]贺晨帮,宗绪晓.豌豆种质资源形态标记遗传多样性分析[J].植物遗传资源学报,2011,12(1):42-48.[3]B0TSTEIN D,WHITE R L,SKOLNICK M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American Journal of Human Genetics,1980,32(3);223-225[4]马明,武天龙.豌豆分子标记研究进展[J].上海交通大学学报(农业科学版),2006(5):489-493.[5]何光华,王文明,刘国庆,等.利用SSR标记定位明恢63的2对恢复基因[J].遗传学报,2002(9):798-802.[6]许占友,邱丽娟,常汝镇,等.利用SSR标记鉴定大豆种质[J].中国农业科学,1999(S1):40-48.[7]〖JP2〗王红梅,张正英,陈玉梁.SSR标记技术及其在植物遗传学中的应用[J].西北师范大学学报(自然科学版),2003(1):113-116.〖JP〗[8]张增翠,侯喜林.SSR分子标记开发策略及评价[J].遗传,2004(5):763-768.[9]GUPTA P K，VARSHNEY R K.The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat[J].Euphytica，2002，113:163-185.[10]宗绪晓,关建平,王述民,等.国外栽培豌豆遗传多样性分析及核心种质构建[J].作物学报,2008(9):1518-1528.[11]顾竟,李玲,宗绪晓,等.豌豆种质表型性状SSR标记关联分析[J].植物遗传资源学报,2011,12(6):833-839.[12]闵学阳,韦兴燚,刘文献,等.箭筈豌豆品种间遗传差异的SSR分析及指纹图谱构建[J].草业学报,2019,28(4):116-128.[13]侯万伟,刘玉皎.豌豆基因组SSR标记在蚕豆中的通用性分析[J].湖北农业科学,2012,51(1):185-187.[14]邹琦.植物生理学实验指导[M].北京：中国农业出版社，2000.[15]李润丰,赵希艳,高亚弟.2,6-二氯靛酚反滴定法测定红色果蔬中还原型VC[J].营养学报,2012,34(5):507-509.[16]SUE P,GRANT B,BERNARD R.Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components[J].Plant Molecular Biology Reporter,1997,15(1):34-37.[17]ANDREW H，PATERSON,CURT L，et al.A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis[J].Plant Molecular Biology Reporter,1993,11(2):48-49.[18]孙雪莲.豌豆SSR标记开发及遗传连锁图谱构建[D].北京：中国农业科学院,2013.[19]YEH F C，YANG R C，BOYLE T.Popgene version 1.31 quick user guide[M].Canada：University of Alberta，and Centre for International Forestry Research，1999.[20]ROHLF.NTSYS-pcver.2.2：Numerical taxonomy system[M].Setauket，New York：Exeter Publishing Ltd.，2006.[21]侯万伟,严清彪,张小田,等.西北地区主栽豌豆品种SSR指纹图谱构建[J].西南农业学报,2012(1):252-254.[22]宗绪晓,REBECCA F,ROBERT R R,等.豌豆属(Pisum)SSR标记遗传多样性结构鉴别与分析[J].中国农业科学,2009(1):44-54.[23]宗绪晓,关建平,王述民,等.中国豌豆地方品种SSR标记遗传多样性分析[J].作物学报,2008(8):36-44.[24]JAI C R,MANEET R,VIKAS S,et al.Genetic diversity and structure of pea (Pisum sativum L.) germplasm based on morphological and SSR markers[J].Plant Molecular Biology Reporter,2017,35(1):48-50.[25]AMIN M,SANTIAGO M，LOUMEREM,et al.Assessment of genetic diversity among local pea (Pisum sativum L.) accessions cultivated in the arid regions of Southern Tunisia using agro-morphological and SSR molecular markers[J].Genetic Resources and Crop Evolution,2019,66(6)：27-29.[26]SUJINNA D,PRAKIT S,SUPALAK P,et al.Genetic diversity and structure of the zombi pea (Vigna vexillata (L.) A.Rich) gene pool based on SSR marker analysis[J].Genetica,2017,145(2):57-59[27]GONG Y M,XU S C,MAO W H,et al.Developing new SSR markers from ESTs of pea(Pisum sativum L.)[J].Journal of Zhejiang University-Science B(Biomedicine & Biotechnology),2010,11(9):702-707.[28]SUN X L,YANG T,HAO J J，et al.SSR genetic linkage map construction of pea (Pisum sativum L.) based on Chinese native varieties[J].The Crop Journal,2014,2(2-3):33-34.[29]郁香荷,章秋平,刘威生,等.中国李种质资源形态性状和农艺性状的遗传多样性分析[J].植物遗传资源学报,2011,12(3):402-407.[30]李玲.国内豌豆种质资源形态性状多样性分析[D].北京：中国农业科学院,2009.

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Last Update: 2021-05-10