|Table of Contents|

Establishment of in vitro Direct Regeneration System on Virus-free Bulblets of ‘Sorbonne’

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年09
Page:
80-86
Research Field:
Publishing date:

Info

Title:
Establishment of in vitro Direct Regeneration System on Virus-free Bulblets of ‘Sorbonne’
Author(s):
LI Xueyan; HU Xinying; WANG Weidong; BAI Yiguang; YANG Yingdong
(Institute of Flowers，Liaoning Academy of Agricultural Science，Shengyang，Liaoning 110161)
Keywords:
Lilium; virus-free; bulblets in vitro; direct regeneration; breeder′s stock
PACS:
-
DOI:
10.11937/bfyy.20201935
Abstract:
Taking ‘Sorbonne’ bulbs as test material,the ‘Sorbonne’ bulbs without CMV，LSV and LMoV virus were obtained by RT-PCR.Scales excised of in vitro stock were used as secondary explants to explore the effects of different plant growth regulators on the direct regeneration of bulblets in vitro,in order to provide reference for breading high-quality virusfree lily bulbs.The results showed that the optimal medium for direct regeneration of bulblets in vitro was MS+2，4-D 1.0 mg?L-1，and the induction rate and induction coefficient were 100% and 3.69，respectively.The optimal medium for small bulb enlargement was MS+sucrose 90 g?L-1，MS+NAA 0.1 mg?L-1 was best for rooting，and the regenerated plantlets survived of ‘Sorbonne’ with highest rate of 100.00%.

References:

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Last Update: 2021-08-06