|Table of Contents|

Extraction of Total Flavonoid in Taxus media by Response Surface Methodology and Its Antioxidant Activity

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年05
Page:
94-102
Research Field:
Publishing date:

Info

Title:
Extraction of Total Flavonoid in Taxus media by Response Surface Methodology and Its Antioxidant Activity
Author(s):
LI Yanyan1; 2; WANG Qianqian1; SU Yuanyuan1; XU Xingxing1; WANG Junqing1; 2
(1.Pingdingshan University,Pingdingshan Medicinal Plant Functional Component Utilization Engineering Technology Research Center,Pingdingshan,Henan 467000;2.Henan Funiu Mountain Endangered Plant Breeding Engineering Technology Research Center,Pingdingshan,Henan 467000)
Keywords:
Taxus media; antioxidant activity; thermal reflow; response surface methodology
PACS:
-
DOI:
10.11937/bfyy.20201562
Abstract:
Taking the branches and leaves of Taxus media as experiment materials,on the basis of single factor experiment,the response surface method was used to optimize the thermal reflux ectraction process of total flavonoid from Taxus media,and the scavenging rate of DPPH?,ABTS+? and nitrite radicals were used to study the antioxidant activity of total flavonoid,in order to provide a reference for the further development and utilization of T.media.The results showed that the optimal extraction process was as following,the liquid-material ratio was 32∶1 mL?g-1,ehanol concentration was 63%,extraction temperature was 71 ℃ and extraction time was 86 minutes,the number of extraction was 2 times.Under the above conditions,the extraction yield of total flavonoid from T.media was up to 128.10 mg?g-1.The extraction rate of total flavonoid was higher in extraction process,therefore the extraction technology of total flavonoid from T.media was stable and applicable.At the maximum total flavonoid extract concentration,the scavenging rates of DPPH?,ABTS+? and nitrite were 91.04%,99.17% and 65.50%,respectively.The result indicated that the antioxidant activity from T.media was strong in vitro.

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Last Update: 2021-06-07