|Table of Contents|

Key Techniques in Tissue Culture With Vaccinium bracteatum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年05
Page:
114-118
Research Field:
Publishing date:

Info

Title:
Key Techniques in Tissue Culture With Vaccinium bracteatum
Author(s):
CAI Sheng1; 2; XU Chen1; ZAI Xueming3; CAI Xiaoning1
(1.College of Food Science，Nanjing Xiaozhuang University，Nanjing，Jiangsu 211171;2.College of Agriculture，Nanjing Agricultural University，Nanjing，Jiangsu 210095;3.College of Horticulture，Jinling Institute of Technology，Nanjing，Jiangsu 210038)
Keywords:
Vaccinium bracteatum; tissue culture; adventitious bud elongation; rooting
PACS:
-
DOI:
10.11937/bfyy.20201370
Abstract:
Shoots of Vaccinium bracteatum were used as explants to research the key points in tissue culture，which referring to how to obtain sterile explants，how to generate adventitious buds，and how to rooting,in order to established tissue culture of Vaccinium bracteatum.The results showed that the most effective way to disinfect explants in this experiment was soaking them into 0.1% mercury chloride solution for 10 minutes.It was suitable for inducing adventitious buds of Vaccinium bracteatum in WPM basic medium while it was not suitable for inducing adventitious buds of Vaccinium bracteatum in MS basic medium.The highest induction rate of adventitious buds was 97.7%，and the medium was WPM+ZT 2.0 mg?L-1.The medium in which the potato diced was added significantly promoted the elongation of the cluster adventitious buds，so the medium suitable for the elongation of the adventitious buds was WPM+ZT 1.0 mg?L-1+potato diced 100 g?L-1.The rooting effect of the elongated buds was better with 1/2WPM + NAA 1.0 mg?L-1 + activated carbon 500 mg?L-1medium，a rooting rate of 94.7% could be achieved after 6 weeks.

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Last Update: 2021-06-07