|Table of Contents|

Induction and Differentiation of Trollius chinensis Bunge.

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年04
Page:
107-112
Research Field:
Publishing date:

Info

Title:
Induction and Differentiation of Trollius chinensis Bunge.
Author(s):
FENG Shasha; FAN Cuili; ZHENG Zhixin; DU Mei; SUN Hongsheng
(College of Agriculture and Technology,Hebei North University，Zhangjiakou，Hebei 075000)
Keywords:
Trollius chinensis Bunge.; callus; induction; differentiation
PACS:
-
DOI:
10.11937/bfyy.20201054
Abstract:
Taking the seedlings of Trollius chinensis Bunge.in vitro as test materials，the effects of explant type，basic medium and exogenous hormone on callus induction and adventitious bud regeneration were studied by tissue culture method in order to establish its rapid propagation system.The results showed that the optimal explants were cotyledons from seedlings of Trollius chinensis Bunge.MS medium was the best medium for callus induction.Medium plus 2.0 mg?L-1 6-BA and 1.0 mg?L-1 2，4-D was advantageous to callus induction，and callus rate was 72.00%.Maltose 30 g?L-1 as carbon source was better than sucrose.The medium adding activated charcoal 75 mg?L-1 could prevent brown efficiently.The differentiation medium adding 0.5 mg?L-1 NAA and 1.0 mg?L-1 KT，could improve the differentiation rate of callus.

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Last Update: 2021-06-07