|Table of Contents|

Optimization of Extraction Method and Antioxidant Activities of Total Flavonid From the Leaves of Tussilago farfara L.

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年01
Page:
108-115
Research Field:
Publishing date:

Info

Title:
Optimization of Extraction Method and Antioxidant Activities of Total Flavonid From the Leaves of Tussilago farfara L.
Author(s):
CHEN Sudan; CUI Yunqi; LI Xiuzhen; LI Xueqiang
(College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003)
Keywords:
Tussilago farfara L.; total flavonoid; microwave-assisted extraction; orthogonal test; antioxidant activities
PACS:
-
DOI:
10.11937/bfyy.20200940
Abstract:
The leaves of Tussilago farfara L.were used as test materials,the total flavonoid of which was extracted by microwave-assisted method on the basis of the results of single factor experiment in order to optimize the total flavonoid from the leaves of T.farfara through the orthogonal experiment.Meanwhile,the scavenging effect of extraction on 1,1-diphenyl-2-picrylhydrazyl radical (DPPH?),hydroxyl free radical (?OH) and superoxide anion free radical (O?[TX-*4]2) were measured and determined by spectrophotometry method.The results showed that the optimum extraction parameters were ethanol volume fraction was 70%,microwave extraction time was 3 minutes,ratio of solid to liquid was 1∶15 g?mL-1,microwave power was 500 W.Under these conditions,the extraction rate of total flavonoid from T.farara leaves was 0.91%.In addition,there was a linear relationship between the mass concentration and the antioxidant activity.The clearance rate could be reached to 84.21%,84.10% and 68.32% corresponding to DPPH?,?OH and O?[TX-*4]2，respectively,with the concentration of total flavonoids as 0.069 8 mg?mL-1.Therefore,the total flavonoids from the leaves of T.farfara could have the outstanding antioxidant ability and maybe developed and utilized deeply as the natural antioxidants in the future.

References:

[1]刘毅,王允,万德光,等.款冬花本草考证[J].中药材,2010,33(4):634-636.[2]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015.[3]ADAMCZAK A,OPALA B,GRYSZCZYNSKA A,et al.Content of pyrrolizidine alkaloids in the leaves of coltsfoot (Tussilago farfara L.) in Poland[J].Acta Societatis Botanicorum Poloniae,2013,82(4):289-293.[4]ZHI H J,QIN X M,SUN H F,et al.Metabolic fingerprinting of Tussilago farfara L.using 1H-NMR spectroscopy and multivariate data analysis[J].Phytochem Anal,2012,23(5):492-501.[5]李静,李娟,贾金萍,等.基于UHPLC-Q Extractive轨道阱高分辨质谱的款冬花、叶的化学比较[J].药学学报,2018,53(3):444-452.[6]李娟.款冬花、叶配伍紫菀散的毒性和化学比较[D].太原:山西大学,2017.[7]米霞.药用植物款冬的质量评价及资源利用[D].太原:山西大学,2014.[8]LI J,ZHANG Z Z,LEI Z H,et al.NMR based metabolomic comparison of the antitussive and expectorant effect of Farfarae Flos collected at different stages[J].Journal of Pharmaceutical and Biomedical Analysis,2018,150:377-385.[9]刘可越,张铁军,高文远,等.款冬花的化学成分及药理活性研究进展[J].中国中药杂志,2006,31(22):1837-1841.[10]吴笛,张勉,张朝凤,等.款冬花中黄酮和酚酸类成分的研究[J].中国中药杂志,2010,35(9):1142-1144.[11]CHO J,KIM H M,RYU J,et al.Neuroprotective and antioxidant effects of the ethyl acetate fraction prepared from Tussilago farfara L.[J].Biological and Pharmaceutical Bulletin,2005,28(3):455-460.[12]程晓叶,张霞,廖曼,等.UPLC-Q-TOF-MS法分析款冬花的化学成分[J].中草药,2017,48(12):2390-2400.[13]韩聪聪.款冬花中萜类和黄酮类化合物分离技术研究[D].重庆:重庆大学,2015.[14]王建全,张亚娜,林敏.乙醇/硫酸铵双水相体系分离纯化款冬花总黄酮[J].井冈山大学学报 (自然科学版),2016,37(1):40-45.[15]刘香,曹娟.分光光度法测定不同产地款冬的总黄酮含量[J].贵阳医学院学报,2010,35(6):572-574.[16]LIU C,QIN K,QI Y,et al.Optimization of ultrasonic extraction of total flavonoids from Tussilago farfara L.using response surface methodology[J].Pharmazie,2014,69(4):311-315.[17]刘蓓,高超,邱悦,等.款冬总黄酮提取优化及其降脂抗氧化作用研究[J].食品工业科技,2018,39(20):180-185.[18]傅荣杰,冯怡.微波萃取技术在中药及天然产物提取中的应用[J].中国中药杂志,2003(9):14-17.[19]李月,刘妍.微波法提取款冬总黄酮的研究[J].化学与生物工程,2010,27(11):43-46.[20]翁梁,李西腾.白茅根总黄酮提取工艺及其抗氧化性研究[J].江苏农业科学,2018,46(10):187-189.[21]杨喆,万山,张乔会,等.响应面法优化山杏核壳总黄酮提取工艺及其抗氧化性的研究[J].食品工业科技,2015,36(6):279-284.[22]周林宗,张伦,杨申明,等.刺槐花总黄酮超声辅助提取工艺优化及抗氧化性评价[J].江苏农业科学,2017,45(13):146-150.[23]王慧芳,邵圣娟,王曼,等.陈皮总黄酮提取及抑菌活性初探[J].食品工业科技,2018,39(8):130-135.[24]王晓杰,黄立新,张彩虹,等.微波辅助提取橄榄苦苷及其抗氧化性能研究[J].食品工业科技,2018,39(7):140-145.[25]姚新鼎,姜飞燕,王玉伟,等.微波辅助提取山竹壳中的总黄酮及其体外抗氧化活性研究[J].食品研究与开发,2019,40(4):114-118.[26]战英,陈丽娜,石矛.微波辅助提取红松松针工艺优化[J].食品研究与开发,2015,36(16):69-72.[27]刘桂萍,崔博籍,孙久阳,等.微波辅助提取杜仲叶总黄酮工艺优化[J].中国酿造,2015,34(5):127-130.[28]田栋.款冬止咳化痰活性成分群及转录组研究[D].太原:山西大学,2015.[29]王彦平,汤高奇,孙瑞琳,等.微波辅助提取葡萄皮渣总黄酮及其抗氧化研究[J].食品研究与开发,2017,38(2):51-55.[30]崔鹏,陈志冉,史雷革.微波提取无花果叶中的总黄酮及其体外抗氧化活性研究[J].食品研究与开发,2018,39(1):41-45.

Memo

Memo:
-
Last Update: 2021-04-21