|Table of Contents|

Study on Callus Induction and Proliferation Culture System of Prunus domestica L.

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年24
Page:
1-7
Research Field:
Publishing date:

Info

Title:
Study on Callus Induction and Proliferation Culture System of Prunus domestica L.
Author(s):
CHEN Xi; XIE Ganggang; LIU Youxian; JING Jianyong; MA Baiqiang; GENG Wenjuan
(College of Forestry and Horticulture,Xinjiang Agricultural University,Urumqi,Xinjiang 830052)
Keywords:
Prunus domestica L.; leaves; callus; tissue culture
PACS:
-
DOI:
10.11937/bfyy.20200750
Abstract:
The leaves of Prunus domestica L.were used as experimental materials in this study.The factors affecting callus induction and proliferation of Prunus domestica L.leaves,such as disinfection time,dark treatment time,type of basic medium,type and concentration of growth regulators and so on,were screened.In order to provide a basis for establishing a highly efficient culture system of Prunus domestica L..The results showed that disinfection time 70% alcohol treatment 40 seconds+0.1% mercuric chloride 6 minutes treatment,the lowest contamination rate of leaves was 6.67%,and the mortality rate was 0%.After 28 days of dark treatment,the callus induction rate of leaves was up to 80.5%,and the leaf veins swelled significantly and dedifferentiated to form calli.The maximum callus induction rate of leaves in B5 medium was 75.56%.The maximum callus induction rate of leaves formed by growth regulators was 86.67%.The optimum culture method for callus induction of Prunus domestica L. leaves was to disinfect 40 seconds with 70% alcohol and wash it with sterile water for 2-3 times.Then soak in 0.1% mercuric chloride solution for 6 minutes and wash it with sterile water for 4-5 times.The best dark treatment time was 14 days.The better medium formula for primary culture was B5+7.5 mg?L-1 TDZ+2.5 mg?L-1 IBA.

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Last Update: 2021-02-19