|Table of Contents|

Screening and Identification of Walnut Bacterial Blight

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年23
Page:
10-16
Research Field:
Publishing date:

Info

Title:
Screening and Identification of Walnut Bacterial Blight
Author(s):
TU Chang1; 2; KE Wenshan2; ZOU Lulu1; 2; ZHANG Cheng1; FU Benzhong1; WANG Lihua1
(1.Hubei Key Laboratory of Quality and Safety Control of Characteristic Fruits and Vegetables,Hubei Engineering University,Xiaogan,Hubei 432000；2.College of Life Sciences,Hubei University,Wuhan,Hubei 430062)
Keywords:
endophytic bacteria; bacterial blight of walnut; antagonism; screening; identification
PACS:
-
DOI:
10.11937/bfyy.20200670
Abstract:
In this study,including loquat,eggplant and metasequoia,45 kinds plants healthy leaves or stems were used as experiment materials,80 strains of endophytic bacteria were isolated.By using plate-confrontation assay,the interactions were studied between the isolated strains and the walnut pathogenic bacterium Xanthomonas arboricola pv.juglandis,respectively,in order to provide theoretical basis for the biological control of walnut bacterial blight.The results showed that five of the isolated,PPE17-18,QCE17-2,QZYE-41,QZJ-42 and SSE17-24,had obvious antagonistic effects.Among them,QZYE-41 had the strongest antibacterial ability,while PPE17-18 and SSE17-24 had weaker antibacterial ability.All the five strains were gram-positive bacteria.Through 16S rDNA and gyrB gene sequencing analysis,combined with colony morphology and physiological and biochemical tests,it was preliminarily determined that both QZYE-41 and SSE17-24 were Bacillus subtilis,PPE17-18 and QZJ-42 were Acinetobacter baumannii,and QCE17-2 was Acinetobacter sp.This study provided a potential new material for the developing and application for biological control of walnut bacterial blight.

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Last Update: 2021-02-18