|Table of Contents|

Screening and Identification of High Efficient Phosphate-solubilizing Bacteria From Vermicompost

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年24
Page:
76-81
Research Field:
Publishing date:

Info

Title:
Screening and Identification of High Efficient Phosphate-solubilizing Bacteria From Vermicompost
Author(s):
LI Hui1PANG Jianzhou2WEI Shuzhen1WANG Qian3CUI Enxi3ZHANG Zhuchen3
(1.Centre for Wetland Conservation and Research,University of Hengshui,Hengshui,Hebei 053000;2.Institute of Dry Farming,Hebei Academy of Agriculture and Forestry Sciences,Hengshui,Hebei 053000;3.Department of Biology,University of Hengshui,Hengshui,Hebei 053000)
Keywords:
phosphate-solubilizing bacteriaidentificationsoluble phosphorusphosphate solubilizing power
PACS:
-
DOI:
10.11937/bfyy.20200631
Abstract:
The earthworm manure was used as the material in this study.Seven strains of phosphate bacteria LH01,LH02,LH03,LH04,LH05,LH06,and LH07 were isolated and screened by selective medium primary screening and molybdenum-antimony anticolorimetry.Among them, the LH05 bacteria had the strongest ability to dissolve phosphorus, and the ratio of the inner and outer diameters of the phosphate ring was 2.26.According to the results of morphological characteristics,physiological and biochemical identification and 16S rDNA homology analysis,the bacterium was preliminarily identified as Bacillus pumilus.The results showed that orthogonal experiment,optimum conditions of phosphorus removal of LH05 were 9% inoculum amount,35 ℃ culture temperature,initial pH 6.5 and 96.69 mg?L-1 dissoluble phosphorus content.Which provided stable and high-efficient bacteria and theoretical basis for further development of high-efficiency phosphorus removal bacteria.

References:

[1]唐岷宸,李文静,宋天顺,等.一株高效解磷菌的筛选及其解磷效果验证[J].生物技术通报,2020,36(6):1-8.[2]王艳霞,解志红,张蕾,等.田菁根际促生菌的筛选及其促生耐盐效果[J].微生物学报,2020,60(5):1023-1035.[3]SAURABH J S,SATINDER K B,YANN L B,et al.Potential application of biohydrogen production liquid waste as phosphate solubilizing agent:A study using soybean plants[J].Appl Biochem Biotechnol,2016,178:865-875.[4]严漪云.污泥蚓粪对滩涂土壤重要理化性质的影响及培肥效应[D].扬州:扬州大学,2019.[5]严漪云,左文刚,徐凯达,等.污泥蚓粪对滩涂盐碱地土壤的培肥效应[J].扬州大学学报(农业与生命科学版),2019(3):111-116.[6]张孟豪,吴家龙,张池,等.赤子爱胜蚓对赤红壤铝形态的影响[J].华南农业大学学报,2020,41(2):48-54.[7]韩雪娇,曾庆伟,赵玉萍.杨树根际解无机磷细菌Mp1-Ha4的鉴定及其解磷机理[J].生物技术通报,2020,36(3):141-147[8]向万胜,黄敏,李学垣,等.土壤磷素的化学组分及其植物有效性[J].植物营养与肥料学报,2004,10(6):663-670.[9]周德庆.微生物学实验手册[M].上海:上海科学技术出版社,1986.[10]东秀珠.常见细菌系统鉴定手册[M].北京:北京科学出版社,2001.[11]梁国栋.最新分子生物学实验技术[M].北京:科学出版社,2001.[12]韩蕾,杨乐,唐金铭,等.解磷菌发酵及溶磷条件的研究[J].生物技术通报,2019(1):98-104.[13]李海云,牛世全,孔维宝,等.猪粪堆肥中一株溶磷菌的筛选、鉴定及溶磷能力初步测定[J].环境科学学报,2015,35(5):1464-1470.[14]苏辉兰,莫雪雪,余炳锋,等.贡柑果园高效解磷菌的筛选及其解磷条件优化[J].北方园艺,2019(5):101-107.[15]吴海波,王樱潼,叶维雁,等.4株番木瓜根际解磷细菌发酵条件优化研究[J].西南林业大学学报(自然科学版),2018,38(2):117-125.[16]LIU F P,LIU H Q,ZHOU H L,et al.Isolation and charac-terization of phosphate-solubilizing bacteria from betel nut (Arecacatechu) and their effects on plant growth and phosphorus mobilization in tropical soils[J].Biol Fertil Soils,2014,50:927.[17]杜雷,王素萍,陈钢,等.一株高效解磷细菌的筛选、鉴定及其溶磷能力的研究[J].中国土壤与肥料,2017(3):136-141.[18]李学平,谢文军,范延辉,等.盐碱地塔宾曲霉菌的解磷能力及其对小麦生长的影响[J].水土保持通报,2017,37(1):93-96.[19]柯春亮,陈宇丰,周登博,等.香蕉根际土壤解磷细菌的筛选、鉴定及解磷能力[J].微生物学通报,2015,42(6):1032-1042.[20]李海峰,张月阳,曹健,等.耐寡营养高效解磷菌株的分离鉴定及解磷特性[J].河南农业科学,2017,46(8):67-71.[21]王样庭,陈玲,王陶,等.牛蒡根际土壤解磷菌的筛选、鉴定及生长特性[J].河南农业科学,2018,47(10):57-63.

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Last Update: 2021-03-29