|Table of Contents|

Cloning and Expression Analysis of ClERF1 Gene in Chrysanthemum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年01
Page:
66-72
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of ClERF1 Gene in Chrysanthemum
Author(s):
REN Manrong; QUAN Yingjie; YUE Yuanyuan; YANG Wenting; HE Zihan; GAO Ri
(College of Agriculture,Yanbian University,Yanji,Jilin 133002)
Keywords:
chrysanthemum; AP2/ERF; gene cloning; promoter element
PACS:
-
DOI:
10.11937/bfyy.20200582
Abstract:
The leaves of chamomile were used as the test material,RT-PCR,Tail-PCR and bioinformatics methods were used to study the characteristics and expression patterns of the promoter sequences of ClERF1 and its response to low temperature stress.In order to provide a theoretical basis for further study on the function of chamomile ClERF1.The results showed that ClERF1 gene sequence was 1 242 bp,with a maximum open reading frame (ORF) of 618 bp,encoding 206 amino acids.Manager property analysis showed that ClERF1 molecular weight 23 631.35 Da,theoretical pI 5.19,instability index 53.84,aliphatic index 62.04,grand average of hydrophilicity -0.786,and subcellular location in the nucleus.Phylogenetic tree showed that ClERF1 was closely related to arabidopsis At3g23240 and belonged to the ERF subfamily.qRT-PCR analysis showed that ClERF1 expressed the highest expression in leaves,followed by in stem segments.ClERF1 promoter sequence of 1 534 bp was cloned,which mainly included low temperature reaction and ethylene response element,auxin and methyl jasmonate reaction element.The expression level of ClERF1 in chamomile seedlings treated at different low temperatures was significantly higher than that in the seedlings not treated at low temperatures,and the relative expression level of ClERF1 was the highest at 5 ℃.

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Last Update: 2021-04-08