|Table of Contents|

Effects of Chaetomium globosum ND35 on Germination and Radicle Growth of Cucumber Seeds

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年23
Page:
1-9
Research Field:
Publishing date:

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Title:
Effects of Chaetomium globosum ND35 on Germination and Radicle Growth of Cucumber Seeds
Author(s):
ZHANG Rui1; TIAN Yehan1; LI Chao2; HE Bangling1; GAO Kexiang1
(1.College of Plant Protection,Shandong Agricultural University/Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests,Tai′an,Shandong 271018;2.Forestry Farm of Culai Mountain of Tai′an City,Tai′an,Shandong 271000)
Keywords:
Chaetomium globosum ND35; cucumber; seed germination; endogenous hormone; enzyme activity
PACS:
-
DOI:
10.11937/bfyy.20200554
Abstract:
‘Jinchun No.4’ cucumber seed was used as experimental materials,the double filter paper culture method was used,the concentrations（1×104 cfu·mL-1,1×105 cfu·mL-1,1×106 cfu·mL-1,1×107 cfu·mL-1,1×108 cfu·mL-1 of Chaetomium globosum ND35 spore suspension were used to treat cucumber seeds.Effects of different concentrations of C.globosum ND35 spore suspension treatment on seeds germination of cucumber and radicle growth were investigated,and the optimum concentration (1×106 cfu·mL-1) of C.globosum ND35 spore suspension on the protective enzyme activity,reactive oxygen content and endogenous hormones content during cucumber seeds germination were furtherly studied,in order to provide a reference for the application of C.globosum ND35 in cucumber seedlings.The results showed that 1×104-1×106 cfu·mL-1 of C.globosum ND35 spore suspension could promote the germination and radicle growth of cucumber seeds,and the promotion effect increases with the increasing of concentration,however 1×107-1×108 cfu·mL-1 of C.globosum ND35 spore suspension could inhibited germination and radicle growth of cucumber seeds.And 1×106 cfu·mL-1 of C.globosum ND35 spore suspension treatment on the germination potential,germination index,vitality index,radicle length and fresh weight of cucumber seeds was the best,which increased by 56.94%,19.92%,100.12%,66.89% and 449.07%,compared with CK.Cultivation of cucumber seeds treated with C.globosum ND35 spore suspension (1×106 cfu·mL-1) for 48 hours,the germination rate reached 100%,and the root length was significantly higher 117.18% than CK.1×106 cfu·mL-1 of C.globosum ND35 spore suspension could increased the activities of peroxidase (POD),phenylalanine ammonia-lyase (PAL) and plant reactive oxygen species (ROS),hydrogen oxide (H2O2),and endogenous hormones gibberellin (GA),salicylic acid (SA),indole-3-acetic acid (IAA),jasmonic acid (JA) were all increased varying degrees.

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Last Update: 2021-02-18