|Table of Contents|

Changes in Enzymatic Activity of Different Flowering Stages of Phalaenopsis aphrodite Cut-flower

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年02
Page:
85-90
Research Field:
Publishing date:

Info

Title:
Changes in Enzymatic Activity of Different Flowering Stages of Phalaenopsis aphrodite Cut-flower
Author(s):
JIANG Suhua1ZHANG Yan1NIU Suyan1YANG Xudong1WANG Ximeng2CUI Bo1
(1.Institute of Bioengineering,Zhengzhou Normal University,Zhengzhou,Henan 450044;2.College of Life Sciences,Zhengzhou University,Zhengzhou,Henan 450002)
Keywords:
Phalaenopsis aphroditeperoxidasesuperoxide dismutaseneutral protease
PACS:
-
DOI:
10.11937/bfyy.20200456
Abstract:
Taking fresh cut-flower of Phalaenopsis aphrodite as test materials,the changes in the activities of the three enzymes at different stages of the cut-flower tepals were measured and analyzed,in order to provide reference for prolonging the life span cut-flower of Phalaenopsis aphrodite.The results showed that the enzyme activity of peroxidase in sepals and petals did not change significantly from the bud stage to the half-blooming stage,and the enzyme activity increased significantly from the half-blooming stage to the senescence stage.The enzyme activity of superoxide dismutase in petals increased from the bud stage to the half-blooming stage,and the enzyme activity decreased significantly from the half-blooming stage to the senescence stage.The enzyme activity of neutral protease in the petals shows an upward trend from the bud stage to the half-blooming stage,a downward trend from the half-blooming stage to the blooming stage,and an upward trend from the blooming stage to the senescence stage.However,In the sepals the enzyme activity of the neutral protease showed a downward trend from the bud stage to the blooming stage,and an upward trend from the blooming stage to the senescence stage.Overall,the enzyme activity of neutral protease in both sepals and petals showed a first decline and then an upward trend.

References:

[1]张永平.蝴蝶兰花自然衰老与活性氧代谢的关系[J].东北林业大学学报,2013,41(3):78-81.[2]顾雯雯,胡亚婷,韩英,等.植物过氧化物酶同工酶的研究进展[J].安徽农业科学,2014,42(34):12011-12013.[3]张维静,陆海,杜希华.抗坏血酸过氧化物酶在植物抵抗氧化胁迫中的作用[J].山东师范大学学报(自然科学版),2008,23(4):113-115.[4]GIACOMELLI L,MASI A,RIPOLL D R,et al.Arabidopsis thaliana deficient in two chloroplast ascorbate peroxidases shows accelerated light-induced necrosis when levels of cellular ascorbate are low[J].Plant Molecular Biology,2007,65(5):627-644.[5]田国忠,李怀方,裘维蕃.植物过氧化物酶研究进展[J].武汉植物学研究,2001(4):332-344.[6]van BUER J,CVETKOVIC J,BAIER M.Cold regulation of plastid ascorbate peroxidases serves as a priming hub controlling ROS signaling in Arabidopsis thaliana[J].BMC Plant Biology,2016,16(1):163.[7]窦俊辉,喻树迅,范术丽,等.SOD与植物胁迫抗性[J].分子植物育种,2010,8(2):359-364.[8]袁牧,王昌留,王一斐,等.SOD的研究进展[J].中国组织化学与细胞化学杂志,2016,25(6):550-558.[9]MARQUES A T,SANTOS S P,ROSA M G,et al.Expression,purification and crystallization of MnSOD from Arabidopsis thaliana[J].Acta Crystallographica,2014,70(5):669-672.[10]冯坤,郑青松,俞佳虹,等.超氧化物歧化酶的遗传特征及其在植物抗逆性中的研究进展[J].分子植物育种,2017,15(11):4498-4505.[11]夏民旋,王维,袁瑞,等.超氧化物歧化酶与植物抗逆性[J].分子植物育种,2015,13(11):2633-2646.[12]AN Y,SHEN Y B,ZHANG Z X.Effects of mechanical damage and herbivore wounding on H2O2 metabolism and antioxidant enzyme activities in hybrid poplar leaves[J].Journal of Forestry Research,2009,20(2):156-160.[13]ROLAND H,CHRISTIAN W G.Peptide-based protease inhibitors from plants[J].Drug Discovery Today,2019,24(9):1877-1889.[14]汪湲.水稻叶片衰老过程生理变化及蛋白质降解与蛋白酶活性变化研究[D].扬州:扬州大学,2010.[15]王凤兰,张昭其.切花花瓣衰老机理研究进展[J].浙江农业学报,2011,23(5):1060-1068.[16]徐萌,刘清岱,朱晔荣,等.植物蛋白酶研究进展[J].生物学通报,2008(6):7-9.[17]KOSKELA E A,MOUHU K,ALBANI M C,et al.Mutation in TERMINAL FLOWER 1 reverses the photoperiodic requirement for flowering in the wild strawberry Fragaria vesca[J].Plant Physiology,2012,159(3):1043-1054.[18]吴志成,黄立新,胡耀明,等.纳米银预处理对非洲菊切花抗氧化酶活性的影响[J].仲恺农业工程学院学报,2012,25(1):16-19.[19]杨秋生,籍越,何松林,等.郁金香切花衰老过程中内源乙烯和SOD的变化[J].河南农业大学学报,1995(4):324-327.[20]刘亚杰.香雪兰切花瓶插期间的生理生化特性研究[D].上海:上海交通大学,2014.[21]ALAEY M,BABALAR M,NADERI R,et al.Effect of pre-and postharvest salicylic acid treatment on physio-chemical attributes in relation to vase-life of rose cut flowers[J].Postharvest Biology and Technology,2011,61(1):91-94.[22]黄浩宇,肖云骁,石乐松,等.文心兰切花花被不同开放阶段酶活性的变化[J].热带生物学报,2016,7(2):199-203.[23]刘雅莉,王荣花,李京春.两个牡丹切花品种低温贮藏过程中抗氧化酶活性的变化[J].园艺学报,2005(6):1114-1117.

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Last Update: 2021-05-19