|Table of Contents|

Isolation and Identification of Root Rot Pathogens on Paeonia suffruticosa Andr. in Luoyang

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年01
Page:
59-66
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Root Rot Pathogens on Paeonia suffruticosa Andr. in Luoyang
Author(s):
YANG Ruixian; LIU Ping; WANG Yuting; FENG Wenjing; HUANG Xuecheng; WANG Yudie
(College of Environmental Engineering and Chemistry,Luoyang Institute of Science and Technology,Luoyang,Henan 471023)
Keywords:
Paeonia suffruticosa Andr.; root rot; pathogenicity; Fusarium solani; Fusarium oxysporum
PACS:
-
DOI:
10.11937/bfyy.20200399
Abstract:
In order to identify the pathogens of root rot of tree peony,infected roots were used as test materials from different cultivated fields in Luoyang from 2016 to 2018.The pathogenic isolates were obtained and purified by tissue isolation methods.The pathogenicity was proved by the Koch′s rule.The pathogenic isolates were identified based on morphological characteristics and gene sequencing analysis.The results showed that fifteen isolates were obtained from 30 samples of root rot.The results of pathogenicity test showed that nine isolates could infect tree peony roots.The pathogens isolates were identified preliminary as Fusarium based on morphological characteristics.The results of rDNA-ITS and [STBX]EF-1α[STBZ] gene sequencing analysis showed that seven isolates were identified as Fusarium solani and two isolates were identified as Fusarium oxysporum.Based on the above results，it was concluded that root rot of tree peony was caused by both F.solani and F.oxysporum in Luoyang,and F.solani was dominant pathogen.

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Last Update: 2021-04-08