|Table of Contents|

Cloning and Expression Analysis of BrTFIIS Gene in Chinese Cabbage

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年24
Page:
33-38
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of BrTFIIS Gene in Chinese Cabbage
Author(s):
QI Xianhui1; WANG Xiaojun2; LI Gaizhen1; WU Dongtang1; ZHAO Junliang1; WANG Xiuying1
(1.Institute of Vegetables,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030031;2.Shanxi Vegetable Industry Management Station,Taiyuan,Shanxi 030002)
Keywords:
Brassica rapapekinensis; BrTFIIS; gene cloning; expression analysis
PACS:
-
DOI:
10.11937/bfyy.20200317
Abstract:
The stem tip growth point of Chinese cabbage was used as the research material,BrTFIIS gene was cloned by RT-PCR from the shoot apex of Chinese cabbage,and its bioinformatics analysis and expression analysis were carried out,in order to provide reference for further study on the flowering mechanism of Chinese cabbage.The results showed that the open reading frame of BrTFIIS contained 1 008 bp and encoded 335 amino acids.The molecular weight of protein was 37.61 kDa，and the isoelectric point was 9.10.Phylogenetic tree analysis indicated that BrTFIIS had the closest relationship with the homologous protein of Brassica napus.The results of real-time PCR showed that BrTFIIS was expressed in the shoot apex of Chinese cabbage at vegetative and reproductive stages.The expression decreased significantly after vernalization,and maintained a low level in the process of flower bud differentiation.BrTFIIS might have an inhibitory effect on vernalization and flower bud differentiation of Chinese cabbage.

References:

[1]BURLE I,DEAN C.The timing of developmental transitions in plants[J].Cell,2006,125(4):655-664.[2]SRIKANTH A,SCHMID M.Regulation of flowering time:All roads lead to Rome[J].Cellular and molecular Life Sciences,2011,68(12):2013-2037.[3]张艺能,周玉萍,陈琼华,等.拟南芥开花时间调控的分子基础[J].植物学报,2014,49(4):469-482.[4]舒黄英,郝圆圆,蔡庆泽,等.模式植物拟南芥开花时间分子调控研究进展[J].植物科学学报,2017,35(4):603-608.[5]王宁.外源H2O2调控拟南芥花发育过程中转录组和DNA甲基化分析[D].河南:郑州大学,2019.[6]李改珍.大白菜春化相关基因筛选及候选基因功能验证[D].山西:山西农业大学,2019.[7]KETTENBERGER H,ARMACHE K J,CRAMER P.Complete RNA polymerase II elongation complex structure and its interactions with NTP and TFIIS[J].Molecular Cell,2004,16(6):955-965.[8]GUGLIELMI B,SOUTOURINA J,ESNAULT C,et al.TFIIS elongation factor and mediator act in conjunction during transcription initiation in vivo[J].Proceedings of the National Academy of Sciences,2007,104(41):16062-16067.[9]GRASSER M,KANE C M,MERKLE T,et al.Transcript elongation factor TFIIS is involved in Arabidopsis seed dormancy[J].Journal of Molecular Biology.2009,386(3):598-611.[10]PEETERS A J,BLANKESTIJN-De V H,HANHART C J,et al.Characterization of mutants with reduced seed dormancy at two novel rdo loci and a further characterization of rdo1 and rdo2 in Arabidopsis[J].Physiologia Plantarum,2002,115(4):604-612.[11]ALKHATEEB W M,SHER A A,MARCUS J M,et al.UVSSA,UBP12,and RDO2/TFIIS contribute to Arabidopsis UV Tolerance[J].Frontiers in Plant Science,2019,10:516.[12]LIU Y,GEYER R,van ZANTEN M,et al.Identification of the Arabidopsis REDUCED DORMANCY 2 gene uncovers a role for the polymerase associated factor 1 complex in seed dormancy[J].PLoS One,2011,6(7):e22241.[13]MORTENSEN S A,SNDERKAER M,LYNGGAARD C,et al.Reduced expression of the [STBX]DOG1[STBZ] gene in Arabidopsis mutant seeds lacking the transcript elongation factor TFIIS[J].FEBS Letters,2011,585(12):1929-1933.[14]宋红霞.普通白菜成花转变基因表达谱比较及BrcLFY基因克隆与表达分析[D].山西:山西农业大学,2015.[15]刘晓柱,李银凤.荠菜生长素极性运输基因1([STBX]CbPIN1[STBZ])的克隆与表达分析[J].华北农学报,2019,34(1):26-32.[16]谢海娟,范希德,叶广继,等.马铃薯[STBX]St4CL[STBZ]的克隆及表达分析[J].生物技术通报,2019,35(11):1-8.[17]包蕊,刘俊频,袁元,等.膜荚黄芪查尔酮还原酶基因的克隆与表达分析[J].北方园艺,2019(6):141-146.[18]张力鹏,韩祥艳,成璐路,等.西藏大花红景天[STBX]cyp450[STBZ]基因的分离与表达分析[J].北方园艺,2019(18):116-127.[19]LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression datausing real-time quantitative PCR and the 2-ΔΔCT method[J].Methods-A Companion to Methods in Enzymology,2001,25(4):402-408.[20]李松洋.大白菜春化种子转录组和DNA甲基化分析[D].沈阳:沈阳农业大学,2017.[21]MORTENSEN S A,GRASSER K D.The seed dormancy defect of Arabidopsis mutants lacking the transcript elongation factor TFIIS is caused by reduced expression of the [STBX]DOG1[STBZ] gene[J].FEBS Letters,2014,588(1):47-51.[22]李曙轩,李式军.白菜的花芽分化与叶球形成[J].中国农业科学,1964(6):36-40.

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Last Update: 2021-03-26