|Table of Contents|

Cloning and Molecular Markers Development of CmFT in Melon（Cucumis melo L.）

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年19
Page:
16-22
Research Field:
Publishing date:

Info

Title:
Cloning and Molecular Markers Development of CmFT in Melon（Cucumis melo L.）
Author(s):
WANG Guichao; WANG Shaozu; LI Zhengzhou; WANG Peng; SHENG Yunyan
(College of Horticulture and Landscape Architecture,Heilongjiang Bayi Agricultural University,Daqing，Heilongjiang 163319)
Keywords:
melon; flowering; CmFT; homologous clone; molecular markers
PACS:
-
DOI:
10.11937/bfyy.20200271
Abstract:
‘HM1-1’ and ‘MS-5’ melon varieties were used as materials，and the melon FT homologous gene was cloned by homologous cloning method，named CmFT.Through phylogenetic tree analysis，real-time fluorescence quantitative methods，and molecular marker-assisted selection breeding methods，the role of CmFT in the development of melon flowers was preliminarily verified.The results showed that the gene was 3 843 bp in length and 534 bp in CDS，encoding 178 amino acids.The accuracy of molecular marker-assisted screening in 24 melon varieties reached 87.5%.The marker was related to the flowering traits of melon and could be used for auxiliary selection in early flowering breeding of 〖JP2〗melon.The results provided a theoretical basis for molecular breeding and flowering gene regulation of melon.

References:

[1]CHARDON F,DAMERVAL C.Phylogenomic analysis of the PEBP gene family in cereals[J].J Mol Evol,2005,61:579-590.[2]孙昌辉,邓晓建,方军,等.高等植物开花诱导研究进展[J].遗传,2007(10):1182-1190.[3]DANILEVSKAYA O N,MENG X,HOU Z,et al.A genomic and expression compendium of the expanded PEBP gene family from maize[J].Plant Physiology,2008,146(1):250-264.[4]BOWMAN J L,ALVAREZ J,WEIGEL D,et al.Control of flower development in Arabidopsis thaliana by 〖STBX〗APETALA1 〖STBZ〗and interacting genes[J].Development,1993,119:721-743.[5]MATSOUKAS I G,MASSIAH A J,THOMAS B.Florigenic and antiflorigenic signaling in plants[J].Plant and Cell Physiology,2012,53(11):1827-1842.[6]XU F,RONG X,HUANG X,et al.Recent advances of Flowering Locus T gene in higher plants[J].International Journal of Molecular Sciences,2012,13(3):3773-3781.[7]JAEGER K E,PULLEN N,LAMZIN S,et al.Interlocking feedback loops govern the dynamic behavior of the floral transition in Arabidopsis[J].The Plant Cell,2013,25(3):820-833.[8]PATIL H B,CHAURASIA A K,AZEEZ A,et al.Characterization of two TERMINAL FLOWER1 homologs PgTFL1 and PgCENa from pomegranate (Punica granatum L.)[J].Tree Physiology,2017,38(5):772-784.[9]WIGGE P A.Integration of spatial and temporal information during floral induction in Arabidopsis[J].Science,2005,309(5737):1056-1059.[10]张娟,颜爽爽,赵文圣,等.黄瓜CsFT基因的克隆及其功能分析[J].园艺学报,2013,40(11):2180-2188.[11]HECHT V,LAURIE R E,VANDER SCHOOR J K,et al.The pea GIGAS gene is a flowering locus T homolog necessary for graft-transmissible specification of flowering but not for responsiveness to photoperiod[J].Plant Cell,2011,23:147-161.[12]FAURE S,HIGGINS J,TURNER A,et al.The FLOWERING LOCUS T-like gene family in barley (Hordeum vulgare)[J].Genetics,2007,176(1):599-609.[13]PIN P A,BENLLOCH R,BONNET D,et al.An antagonistic pair of FT homologs mediates the control of flowering time in sugar beet[J].Science,2010,330:1397.[14]KOJIMA S,TAKAHASHI Y,KOBAYASHI Y,et al.〖STBX〗Hd3a〖STBZ〗,a rice ortholog of the Arabidopsis FT gene，promotes transition to flowering downstream of Hd1 under short-day conditions[J].Plant Cell Physiol,2002,43:1096.[15]DANILEVSKAYA O N,MENG X,HOU Z,et al.A genomic and expression compendium of the expanded PEBP gene family from maize[J].Plant Physiol,2008,146:250-264.[16]KONG F,LIU B,XIA Z,et al.Two coordinately regulated homologs of flowering locus T are involved in the control of photoperiodic flowering in soybean[J].Plant Physiol,2010,154:1220-1231.[17]MOLINERO-ROSALES N,LATORRE A,JAMILENA M,et al.Single flower truss regulates the transition and maintenance of flowering in tomato[J].Planta,2004,218:427-434.[18]刘新宇,刘杨,葛海燕,等.茄子开花相关基因SmFT的克隆和表达分析[J].分子植物育种,2015,13(6):1297-1301.[19]BHLENIUS H,HUANG T,CHARBONNEL-CAMPAA L,et al.CO/FT regulatory module controls timing of flowering and seasonal 〖JP2〗growth cessation in trees[J].Science,2006,312:1040.[20]HATTASCH C,FLACHOWSKY H,KAPTURSKA D,et al.Isolation of flowering genes and seasonal changes in their transcript levels related to flower induction and initiation in apple (Malus domestica)[J].Tree Physiology,2008,28(10):1459-1466.[21]POREBSKI S,BAILEY L G,BAUM B R.Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components[J].Plant Molecular Biology Reporter,1997,15:8-15.[22]张丹,宋海娜,程浩,等.大豆耐低磷相关基因的定位与克隆[J].遗传,2015,37(4):336-343.[23]WANG M,TAN Y G,CAI C P,et al.Identification and expression analysis of phosphatidy ethanolamine-binding protein(PEBP) gene family in cotton[J].Genomics,2019,111(6):1373-1380.[24]HANZAWA Y,MONEY T,BRADLEY D.A single amino acid converts a repressor to an activator of flowering[J].Proceedings of the National Academy of Sciences,2005,102(21):7748-7753.[25]DOI K.Ehd1，a B-type response regulator in rice，confers short-day promotion of flowering and controls FT-like gene expression independently of Hd1[J].Genes & Development,2004,18(8):926-936.[26]王亚琦,孙子淇,郑峥,等.作物分子标记辅助选择育种的现状与展望[J].江苏农业科学,2018,46(5):6-12.

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Last Update: 2020-12-24