|Table of Contents|

Comparative Stem Culture and Embryo Culture of Cerasus yedoensis ‘Somei-yoshino’

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年23
Page:
65-71
Research Field:
Publishing date:

Info

Title:
Comparative Stem Culture and Embryo Culture of Cerasus yedoensis ‘Somei-yoshino’
Author(s):
XU Chenjie; OU Jing
(College of Forestry,Guizhou University,Guiyang,Guizhou 550025)
Keywords:
Cerasus yedoensis ‘Somei-yoshino’; stem segment; embryo culture; cluster buds; growth regulator
PACS:
-
DOI:
10.11937/bfyy.20200224
Abstract:
Cerasus yedoensis‘Somei-yoshino’ was used as material,seeding efficiency of different explant by comparing the induction rate of stem with axillary bud and embryo which used as explant in single bud induction,cluster buds induction was imestigate.And the effect of different plant growth regulators on single bud induction，cluster buds induction and rotting was explored.The results showed that MS medium supplemented with 2.0 mg?L-1 6-BA and 0.1 mg?L-1 IBA showed the highest single bud induction rate (61.29%)using stem with axillary bud as explants.The influence of NAA for embryo induction was not significant.MS medium supplemented with 2.0 mg?L-1 6-BA，0.05 mg?L-1 IBA and 0.05 mg?L-1 NAA showed the highest single bud induction rate (86.02%)using embryo as explants．MS medium with 2.0 mg?L-1 6-BA and 0.1 mg?L-1 IBA exhibited the highest clustered bud proliferation rate (3.73 and 4.09)．1/2MS medium with 0.5 mg?L-1 IBA and 0.1 mg?L-1 NAA was suitable for plantlet rooting,and the rooting rate was 70.97%.The survive rate was above 87.09% when the plantlets were due to be moved to the nursery.The medium with 50% humus,25% sand and 25% roseite was effective for rooting．

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Last Update: 2021-02-19