|Table of Contents|

Optimization of Lentinu sedodes Stock Culture Medium by Orthogonal Test

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年19
Page:
129-133
Research Field:
Publishing date:

Info

Title:
Optimization of Lentinu sedodes Stock Culture Medium by Orthogonal Test
Author(s):
SHI Hongge; WEI Yinchu; BAN Xinhe; LI Jiuying
(Zhumadian Academy of Agricultural Sciences,Zhumadian,Henan 463000)
Keywords:
Lentinu sedodes; orthogonal test; medium; optimiz
PACS:
-
DOI:
10.11937/bfyy.20200127
Abstract:
Using Lentinu sedodes strain L808 as test material，a combination of single factor test and orthogonal test was used to optimize the culture medium of Lentinu sedodes stock culture.The results showed that 90 g?L-1 of sawdust，20 g?L-1 of glucose，18 g?L-1 of agar powder，and 200 g?L-1 of peeled potatoes were the best culture medium for the parent species of Lentinu sedodes stock culture.The stock culture of Lentinu sedodes cultivated with the formula has enhanced mycelial ability to decompose cellulose when used to make the original seed.The mycelium was white，thick and dense，and the growth rate of mycelium was increased by 30.2% compared with the unoptimized parent medium.The quality of culture Lentinu sedodes was significantly improved.The formula of the stock culture was 120 g?L-1 of sawdust，10 g?L-1 of glucose，20 g?L-1 of agar powder，and 190 g?L-1 of peeled potatoes.It was more suitable for the oblique storage of the Lentinu sedodes stock culture.The growth rate of the mycelium was relatively slow，and the mycelium was white and thick，which effectively maintains the viability of the mycelium and prevents the degradation of the variety.

References:

[1]张金霞.中国食用菌菌种学[M].郑州:中国农业出版社,2011.[2]魏银初,班新河．香菇种植能手谈经[M].郑州:中原农民出版社,2018.[3]盛祖嘉.关于菌种退化问题[J]．微生物学通报，1974(4):36-40．[4]吴春玲,吴学谦,郑巧平,等.香菇菌种性能衰退的原因及复壮技术[J]．中国食用菌，2006，25(5):9-11．[5]杨新美.中国食用菌栽培学[M].北京:中国农业出版社,1988.[6]张金霞.食用菌的品种菌种质量及其保持：食用菌的品种菌种质量[J]．中国食用菌，1996，15(2):11-12.[7]林娟,周选围，姚玉峰.鸡腿菇母种培养基筛选研究初报[ J].中国食用菌，1997，17(2):22-24.[8]CHANG S T,MELES P G.Edible mushrooms and their cultivation[M].Boca Raton:CRC Press，2004.

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Last Update: 2020-12-24