|Table of Contents|

Study on Changes of Methylation of ‘Fuji’ Apple With Different Color Phases After Unpacking

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年17
Page:
23-29
Research Field:
Publishing date:

Info

Title:
Study on Changes of Methylation of ‘Fuji’ Apple With Different Color Phases After Unpacking
Author(s):
CHI Xin1; 2; WANG Xinyue1; 2; LIU Xueqing1; KELLER-PRZYBYLKOWICZ Sylwia1; SONG Laiqing1; DU Xiaoyun1
(1.Yantai Academy of Agricultural Sciences，Yantai，Shandong 265500；2.School of Life Sciences,Yantai University,Yantai，Shandong 264005)
Keywords:
‘Fuji’ apple; peel tinted; DNA methylation; MSAP analysis
PACS:
-
DOI:
10.11937/bfyy.20200035
Abstract:
14-year-old ‘Fuji’ apples were used as test materials，using the MSAP technology，the changes of genome-wide methylation of peel DNA during the coloring period of 12 days were analyzed after unpacking about the different color ‘Fuji’ apple，so as to explore the relationship between coloring of ‘Fuji’ apple and genome-wide methylation of peel DNA.The results showed that typeⅡ (the outer half cytosine methylation/CGH methylation) and typeⅢ (inside the methyl/CG methylation) methylation level apparent reached an inflection point on the sixth day after bag removal in MSAP analysis.The methylation level and methylation pattern changed significantly in the both coloring periods(before 6 days was the S1 stage，6 days for S2)，and the methylation level in stage S1 was significantly higher than that in stage S2.The methylation pattern was mainly methylated in the early stage and demethylated in the later stage.It could be seen that demethylation may play an important role in the coloring period of ‘Fuji’ apples after bag unpacking.The results of study on striped ‘Fuji’ and blushed ‘Fuji’ showed that the changes in DNA methylation levels and patterns were more pronounced in striped ‘Fuji’.The results of that the methylation level and pattern change of the ‘Fuji’ apple with striped ‘Fuji’ was more obvious.This study could provide new ideas for the identification of ‘Fuji’ apple with different colors，as well as the breeding of ‘Fuji’ apple and the regulation of coloring.

References:

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Last Update: 2020-11-27