|Table of Contents|

Pectin Decomposition Enzyme Gene Cloning and Expression Analysis in Actinidia arguta Fruit

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年15
Page:
21-26
Research Field:
Publishing date:

Info

Title:
Pectin Decomposition Enzyme Gene Cloning and Expression Analysis in Actinidia arguta Fruit
Author(s):
JIN Hualin; JIANG Mingliang; LI Xu; LIAN Zheng; PIAO Yilong
(Agricultural College,Yanbian University,Yanji，Jilin 133000)
Keywords:
Actinidia arguta; PG; β-gal; PME; gene cloning
PACS:
-
DOI:
10.11937/bfyy.20194625
Abstract:
In order to explore the mechanism of Actinidia arguta fruit softening,Actinidia arguta fruit in different softening stages was used as experimental materials.Polygalacturonase activity (PG),β-galactosidase activity (β-gal) and pectin methylesterase activity (PME) were measured by spectrophotometry.The control gene was cloned by RT-PCR,and the expression at different maturity stages was analyzed.It was found that the activity peaks of PME and β-gal during softening of kiwifruit during the softening period,and the peak activity of PG during the softening period.The gene sequences of PG and β-gal from kiwifruit were successfully cloned.NCBI Blast results showed that the identity with Actinidia chinensis Planch.PG gene and β-gal gene were greater than 95% and 90%,respectively.The semi-quantitative analysis of the PG gene showed that the transcriptional expression of the PG gene was synergistic with the PG activity,indicating that the expression of the PG enzyme gene was regulated at the transcriptional level in A.arguta fruits.The results of β-gal activity peaks are consistent,but they are not consistent with the results of β-gal maintaining a higher enzyme activity during fruit softening,which indicates that the expression of β-gal gene is not only regulated by the transcription level,but also may be regulated by the translation level.

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Last Update: 2020-09-16