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¡¶±±·½Ô°ÒÕ¡·[ISSN:1001-0009/CN:23-1247/S]

Issue:

2020Äê16

Page:

22-28

Research Field:

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Title:

Identification of Mobile mRNAs in ª«Arabidopsis/Nicotiana benthamianaª« Hetero-grafts

Author(s):

DENG Zhuying1; 2; LIANG Dacheng1; 2

(1.Hubei Collaborative Innovation Center for Grain Industry,Yangtze University,Jingzhou,Hubei 434025£»2.Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Education,Yangtze University/Hubei Key Laboratory of Waterlogging Disaster and Wetland Agriculture,Jingzhou,Hubei 434025)

Keywords:

Arabidopsisª«; ª«Nicotiana benthamianaª«; hetero-grafting; RNA-Seq; mobile mRNA

PACS:

-

DOI:

10.11937/bfyy.20194602

Abstract:

ª«Arabidopsis ª«and ª«Nicotiana benthamiana ª«were used as materials,by deploying the micrografting technique and deep-sequencing platform,the possibility of potentially mobile mRNAs betweenª« Arabidopsis ª«scion and ª«Nicotiana ª«rootstock were explored in order to understand the mechanisms of root/shoot communication.The results showed that 824 transcripts belonging to ª«Arabidopsisª« were identified in the Nb rootstock about deep sequencing,suggesting that mRNA might move across the graft union.To further confirm the mobility of the identified ª«Arabidopsisª« transcripts,RT-PCR analyses of 30 high-potential mobile transcripts together with previously identified ª«psbW ª«and ª«RBCS1A ª«transcripts were performed.The amplified PCR fragment for ª«AT1G26630 (elF5A2) ª«gene was further cloned and sequenced,and it was from ª«Nicotiana benthamiana ª«rather than from the scion.Mobile mRNA candidates identified with deep sequencing technique cannot be validated by RT-PCR,thereby presenting concerns about the role of mRNAs in acting as long-distance signaling between shoot and root as previously suggested.

References:

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Last Update: 2020-11-23