|Table of Contents|

Establishment of High Efficient in vitro Regeneration System of Material ‘851’ of Tomato

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年13
Page:
1-8
Research Field:
Publishing date:

Info

Title:
Establishment of High Efficient in vitro Regeneration System of Material ‘851’ of Tomato
Author(s):
ZHU Xuemei; WEI Xiaohong; JIN Baoxia; ZHANG Xiaofang; LIU Fang
(College of Life Science and Technology，Gansu Agricultural University/Gansu Key Lab of Crop Genetic & Germplasm Enhancement/Gansu Provincial Key Lab of Aridland Crop Science,Lanzhou，Gansu 730070)
Keywords:
tomato; explant; concentration screening of IAA; regeneration in vitro system
PACS:
-
DOI:
10.11937/bfyy.20194441
Abstract:
The self-cultivated tomato ‘851’ was used as the test material，and the method of plant tissue culture was used to study the effects of different soaking time,light and dark culture treatment on tomato seed germination and seedling growth status；6-BA was used as the basic medium to study the effects of different IAA concentrations，seedling ages and explants on callus and adventitious bud formation；the rooting medium with different treatments was used to study the effect of adventitious buds on rooting with a view to establishing tomato material ‘851’ efficient in vitro regeneration system.The results showed that the tomato seeds were soaked for 3 hours，and then cultured in the dark for 3 days and then light cultured to obtain the sterile seedlings with the best growth state，and their,germination potential，germination rate and germination index were the highest，89%，93% and 5.20 respectively.Cotyledonary nodes，hypocotyls and cotyledons were explants.IAA concentrations of 0.2，0.3，0.5 mg?L-1 were most beneficial to the formation of callus and adventitious buds，and at the age of 10，14，8 days the induction rate of adventitious buds was 160%，91% and 79%，respectively.The optimal rooting medium for treatment was MS+0.5 mg?L-1 IAA with a rooting rate of 89%.

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Last Update: 2020-09-02