|Table of Contents|

Effects of Drought Stress on Secondary Metabolite Contents and Antioxidant Enzyme Activities in Callus of Polygala tenuifolia Willd．

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年22
Page:
109-116
Research Field:
Publishing date:

Info

Title:
Effects of Drought Stress on Secondary Metabolite Contents and Antioxidant Enzyme Activities in Callus of Polygala tenuifolia Willd．
Author(s):
LEI Ruixiang; YANG Bingyue; GAO Jing; WEI Yanni; HU Benxiang; PENG Liang
(School of Pharmacy,Shaanxi University of Traditional Chinese Medicine/Qinling Chinese Herbal Medicine Application Development Engineering Technology Research Center,Xianyang,Shaanxi 712046)
Keywords:
Polygala tenuifolia Willd．; callus; drought stress; total phenol; total flavonoids; antioxidant enzyme activity
PACS:
-
DOI:
10.11937/bfyy.20194366
Abstract:
Taking Polygala tenuifolia callus as test material,using different concentrations of PEG 6000 (polyethylene glycol) solutions to simulate drought stress,the concentration indicators were 5%,10%,15%,20%,30%,and distilled water was used as a control.The effects of drought stress on the growth,total phenols,total flavonoids,Polygala tenuifolia ketone Ⅲ content and antioxidant enzyme activity of Polygala tenuifolia callus were studied,in order to reveal the mechanism of Polygala tenuifolia callus responding to drought stress and to provide a certain reference for the secondary growth of Polygala tenuifolia callus and the large-scale production of metabolites.The results showed that under different degrees of drought stress,the growth,total phenols,total flavonoids,Polygala tenuifolia ketone Ⅲ content and antioxidant enzyme activity of Polygala tenuifolia callus were quite different.With the increasing of the concentration of PEG 6000 solution,the fresh mass,dry mass,total flavonoid content and POD activity of Polygala tenuifolia callus basically increased first and then decreased;total phenol content,Polygala tenuifolia ketone Ⅲ content basically showed a gradually decreasing trend;SOD activity showed a trend of first decreasing and then increasing;CAT activity basically showing a gradually increasing trend,indicating that a certain degree of drought stress had a certain promoting effect for the growth of callus and the accumulation of its secondary metabolites.

References:

[1]李洁,胡本祥,彭亮,等.不同激素配比对远志愈伤诱导及其黄酮积累的影响[J].中国实验方剂学杂志,2019,25(19):131-137.[2]郭丽丽,郭如洹,秦楠,等.远志提取物的工艺、成分及抗氧化活性[J].北方园艺,2019(13):121-129.[3]王媛媛,彭亮,胡本祥.不同商品等级远志药材的质量分析研究[J].中草药,2017,48(18):3833-3840.[4]庞冰,郝建平,董永军,等.山西野生远志资源及其生长环境研究[J].山西农业科学,2018,46(10):1695-1698.[5]杜庆波.中药远志的化学成分研究概况[J].齐齐哈尔医学院学报,2015,36(27):4159-4160.[6]韩丽丽,李真,管仁伟,等.中药远志的研究进展[J].中国野生植物资源,2010,29(6):1-4,13.[7]曹福麟,胡本祥,彭亮,等.H2O2对远志愈伤组织中总酚总黄酮含量及相关酶活性的影响[J].中国实验方剂学杂志,2019,25(20):153-159.[8]黄璐琦,陈美兰,肖培根.中药材道地性研究的现代生物学基础及模式假说[J].中国中药杂志,2004,29(6):494.[9]赵丽英,邓西平,山仑.活性氧清除系统对干旱胁迫的响应机制[J].西北植物学报,2005(2):413-418.[10]PANT N C,AGARRWAL R,AGRAWAL S.Mannitol-induced drought stress on calli of Trigonellafoenum graecum L.var.RMt-303[J].Indian Journal of Experimental Biology,2014,52(11):1128-1137.[11]程焕欣,梁玉玲,姚红宝.干旱胁迫对甘草愈伤组织总黄酮含量的影响[J].安徽农业科学,2010,38(11):5633-5635.[12]蔡小东,崔航.PEG6000模拟干旱胁迫对柑橘愈伤组织抗氧化酶活性的影响[J].长江大学学报(自然科学版),2013,10(23):14-17,5-6.[13]侯云亮,韩梅,马秀杰,等.聚乙二醇模拟水分对黄芩愈伤组织生长及黄芩苷含量的影响[J].吉林农业大学学报,2018,40(6):694-699.[14]DEMETER Z,KANALAS,PETER,et al.Osmotic stress responses of individual white oak (Quercus section,Quercus subgenus) genotypes cultured in vitro[J].Journal of Plant Physiology,2014,171(2):16-24.[15]彭亮,杨冰月,王媛媛,等.远志种苗的质量分级标准[J].中国实验方剂学杂志,2018,24(17):54-59.[16]赵建国,曲伟红.远志化学成分分析方法研究进展[J].时珍国医国药,2009,20(12):3114-3115.[17]马菁菁,刘斌,罗跃娥.远志化学成分和药理活性的研究进展[J].辽宁中医药大学学报,2009,11(12):161-163.[18]LIN L,YAN L,ZHANG H,et al.Simultaneous analysis of polygala acid,senegenin and 3,6′-disinapoylsucrose in rat plasma by liquid chromatography-tandem mass spectrometry:Application to a pharmacokinetic study after oral administration[J].Biomedical Chromatography,2014,28(5):594-600.[19]王兰兰,张立军,陈贵,等.甘薯愈伤组织对干旱胁迫和盐胁迫的生理反应对比[J].生态学杂志,2006(12):1508-1514.[20]彭亮,杨冰月,张岗,等.干旱胁迫对远志种子萌发及幼苗生长和生理特性的影响[J].西北植物学报,2018,38(4):741-749.[21]柳福智,杨秀艳.无机盐对甘草愈伤组织生长及总黄酮含量的影响[J].草业学报,2017,26(5):118-126.[22]SATO A,HISANAKA ITO A,TAGUCHI T.Yield improvement strategies for the production of secondary metabolites in plant tissue culture:Silymarin from Silybum marianum tissue culture[J].Natural Product Research,2014,28(23):2102-2110.[23]李琰,杨广隶,冯俊涛,等.碳源及植物生长调节剂对雷公藤愈伤组织生长和次生代谢产物含量的影响[J].林业科学,2010,46(9):34-39.[24]彭立新,李德全,束怀瑞.园艺植物水分胁迫生理及耐旱机制研究进展[J].西北植物学报,2002(5):1275-1281.[25]张福生,陈彤垚,王丹丹,等.远志药材商品规格等级与品质的关联性研究进展[J].中草药,2017,48(12):2538-2547.[26]李洁,胡本祥,彭亮,等.水杨酸和茉莉酸甲酯对远志愈伤组织生长和相关酶活性及化学成分的影响[J].中草药,2019,50(12):2976-2982.

Memo

Memo:
-
Last Update: 2021-02-07