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Cloning and Expression Analysis of CHI of Paeonia delavayi

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年22
Page:
79-85
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of CHI of Paeonia delavayi
Author(s):
YUAN Xiaolong1; LI Ziguang2; LU Gang2; LI Yunqin1; WANG Yi1
(1.Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants/Conservation of Rare,Endangered ＆ Endemic Forest Plants,Public Key Laboratory of the National Forestry and Grassland Administration,Yunnan Academy of Forestry and Grassland,Kunming,Yunnan 650204；2.Kunming Haikou Forestfarm，Kunming,Yunnan 650114)
Keywords:
Paeonia delavayi; chalcone isomerase; cloning; gene expression
PACS:
-
DOI:
10.11937/bfyy.20194324
Abstract:
Two full length cDNA of the genes([STBX]PdCHI1 and PdCHI2[STBZ]) encoding chalcone isomerase were identified,carried out bioinformatics analysis and detected gene expression in the different development periods of P.delavayi.To comprehend the cause of color formation of Paeonia delavayi.The results showed that [STBX]PdCHI1[STBZ] gene had 654 bp,encodes 217 amino acids;[STBX]PdCHI2[STBZ] gene has 666 bp,encodes 221 amino acids;the phylogenetic analysis revealed PdCHI1 with Ipomoea purpurea(AAB86474),Citrus sinensis(BAA36552),Glycine max(AAT94360) and Lotus japonicas(BAC53984) clustered one clan belonging to type Ⅰ CHI from higher plants;PdCHI2 with Zea mays(NP_001151452),Glycine max(AAT94362) and Vitis vinifera(XP_002280158) clustered the same clan appertaining type Ⅳ CHI.In the different developmental periods of P.delavayi flower,the expression level of [STBX]PdCHI1[STBZ] gene was the highest at budding stage,reduced sharply at initial flowering stage and full-bloom stage,elevated slightly at blossom falling stage;however,when the flower was developing,the [STBX]PdCHI2[STBZ] gene expression level presented the gradually lower trend.

References:

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Last Update: 2021-02-06