|Table of Contents|

Establishment and Application of Duplex PCR to Detection of Bacterial Spot Disease Resistance Genes Bs2 and Bs3 in Pepper

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年13
Page:
9-15
Research Field:
Publishing date:

Info

Title:
Establishment and Application of Duplex PCR to Detection of Bacterial Spot Disease Resistance Genes Bs2 and Bs3 in Pepper
Author(s):
Establishment and Application of Duplex PCR to Detection of Bacterial Spot Disease Resistance Genes Bs2 and Bs3 in Pepper
(1.Cash Crops Research Institute，Hubei Academy of Agricultural Sciences，Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement，Wuhan，Hubei 430064;2.Kasetsart University，Bangkok，Thailand 10900;3.Hubei Academy of Agricultural Sciences，Wuhan，Hubei 430064)
Keywords:
pepper; Bs2 and Bs3; duplex PCR marker
PACS:
-
DOI:
10.11937/bfyy.20194298
Abstract:
Taking cultivated and wild-type peppers as materials,the construction method of duplex PCR system was used to establish a novel duplex PCR marker for detection of bacterial spot disease (BS) resistance genes Bs2 and Bs3,in order to improve the efficiency of germplasm resource identification and molecular marker-assisted screening of breeding generations.The results showed that the duplex PCR marker for detection of bacterial spot disease (BS) resistance genes Bs2 and Bs3 had rich polymorphism and high stability,when Bs2-For/Bs2-Rev and Bs3-For/Bs3-Rev were used as specific primers pairs and annealing temperature was set at 52 ℃.In addition,the novel duplex PCR system was used to identify the resistance of different pepper species materials to bacterial spot disease,and the results were consistent with the inoculation assay,indicating that the duplex PCR system had high reliability.The application of the duplex PCR system in Bs2 and Bs3 gene could improve breeding selection efficiency.

References:

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Last Update: 2020-09-02