|Table of Contents|

Analysis of Differentially Expressed Gene Profile in the Root of Walnut Under Natural Temperature Regulation in the Field

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年17
Page:
30-38
Research Field:
Publishing date:

Info

Title:
Analysis of Differentially Expressed Gene Profile in the Root of Walnut Under Natural Temperature Regulation in the Field
Author(s):
LIU Yang1; 2; LU Kaizheng1; 2; BIAN Shaojie1; QI Guohui1; 2; ZHANG Xuemei1; 2; GUO Suping1; 2
(1.College of Forestry，Hebei Agricultural University，Baoding，Hebei 071000；2.Research Center for Walnut Engineering and Technology of Hebei，Lincheng，Hebei 053400)
Keywords:
walnut; root; natural temperature regulation; transcriptomes; differentially expressed genes
PACS:
-
DOI:
10.11937/bfyy.20194278
Abstract:
Taking 15-year-old ‘Lyuling’ walnut trees as experimental materials，the effects of different ground temperatures(16.4 ℃，W0；21.7 ℃，W1；27.1 ℃,W2) on gene expression of walnut roots were studied by the high-through transcriptome sequencing technology，in order to explore the functional genes related to temperature regulation and explore the molecular mechanism of response of walnut roots to natural temperature regulation.The up-regulated (down-regulated) genes of W1vsW0，W2vsW0 and W2vsW1 were 430(247)，2 110(2 494) and 2 385(3 015)，respectively.GO enrichment analysis showed that differentially expressed genes of W1vsW0，W2vsW0，W2vsW1 were mainly concentrated in biological processes and molecular functions and the significantly enriched category of the three components were amino sugar catabolic process，single-organism biosynthetic process，chitin catabolic process，glucosamine-containing compound catabolic process，oxidation-reduction processand oxidoreductase activity.But the significantly enriched molecular functions category of W1vsW0 were mostly related to the metabolism of substances，while W2vsW1 were mostly related to the transcription factor.KEGG enrichment analysis illustrated that differentially expressed genes of W1vsW0，W2vsW0，W2vsW1 were annotated 74，118 and 117 KEGG pathways and there were 71 biological pathways jointly annotated，among which，the significantly enriched pathways were biosynthesis of secondary metabolites and phenylpropanoid biosynthesis.Compared with W1vsW0,the significantly enriched pathways of the differentially expressed genes of W2vsW1 increased pathway of alpha-Linolenic acid metabolism.Increased temperature could promote plant metabolism and contribute to carbohydrate accumulation.However，excessive temperature decreases the rate of water transport and a large number of genes related to the alpha-Linolenic acid metabolism acid are expressed，which suggested that alpha-Linolenic acid may play an important role in the response of walnut roots to high temperature stress.

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Last Update: 2020-11-27