|Table of Contents|

Effects of Hormones and Their Ratios on the Establishment of Tissue Culture System for the Seeds of Polygonatum cyrtonema Hua

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年13
Page:
118-123
Research Field:
Publishing date:

Info

Title:
Effects of Hormones and Their Ratios on the Establishment of Tissue Culture System for the Seeds of Polygonatum cyrtonema Hua
Author(s):
ZHONG Can1; LAO Jia2; JIN Jian1; HE Wei2; WANG Weizi1; ZHANG Shuihan1
(1.Institute of Chinese Materia Medica,Hunan Academy of Chinese Medicine,Changsha,Hunan 410013;2.Resgreen Group International Inc.,Changsha,Hunan 410329)
Keywords:
Polygonatum cyrtonema Hua; cytokinin; auxin; seeds; sterilization
PACS:
-
DOI:
10.11937/bfyy.20194082
Abstract:
The seeds of Polygonatum cyrtonema Hua were used as test materials to establish a high-efficient in vitro regeneration system.Effect of sterilization,single hormone on seed germination,and hormone ratio on bud growth and root induction were studied .The results showed that the optimal sterilization was using 8% NaClO for 10-15 minutes and 0.1% HgCl2 for 10-15 minutes was good forreducing pollution rate and germination rate between 55%-65%.The germination rate of seeds harvested in the same year was significantly higher than that of seeds stored for one year.Single hormone was not good for seeds gemination.The combination of 1.0 mg?L-1 6-BA+0.5 mg?L-1 NAA benefited to the elongation of adventitious buds.And 1.0 mg?L-1 IBA+0.5 mg?L-1 NAA was the best combination of hormones for rooting,and the temperature was (26±2)℃ with 2 000 lx light intensity for 12 hours one day.The cultivation period was 8-10 months.This study provided a technical support for seedling and variety improvement of removal of Polygonatum cyrtonema Hua.

References:

[1]刘晓谦,易红,姚丽,等.黄精属植物的研究进展及其开发前景[J].中国药学杂志,2017,52(7):530-534.[2]莫勇生,卢拓方,邱展鸿,等.多花黄精组培苗快速繁殖体系建立研究[J].中国现代中药,2018,20(4):445-449.[3]刘芳源,王钰,开桂青,等.多花黄精组培体系建立及薯蓣皂苷等含量测定[J].生物学杂志,2017,34(6):93-96.[4]刘保财,黄颖桢,赵云青,等.多花黄精种苗繁育技术[J].福建农业科技,2017,48(9):36-38.[5]陈松树,张雪,赵致,等.以叶片为外植体的多花黄精组织培养[J].北方园艺,2018(14):136-142.[6]钟灿,王蕾,刘浩,等.卷丹百合鳞茎高效离体再生体系的研究[J].中国现代中药,2015,17(2):136-140.[7]程秋香.黄精种子胚乳弱化机制的研究[D].杨凌:西北农林科技大学,2016.[8]王占红,蒋花,王瑾,等.不同沙藏处理对黄精种子内贮藏物质及萌发的影响[J].种子,2012,31(2):91-93.[9]张新平,张芳芳,王飞,等.植株成熟度与细胞分裂素对三种兰科植物试管开花与组培增殖的影响[J].南方园艺,2017,28(4):11-16.[10]张宵娟,邓光华,连芳青,等.千日红无菌苗生长及试管开花诱导[J].草业科学,2017,34(11):2245-2253.

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Last Update: 2020-09-09