|Table of Contents|

Identification of Biocontrol Strain KC24 and Preliminary Study on the Control Effect of Fermentation Liquid on Begonia Rust

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年11
Page:
19-24
Research Field:
Publishing date:

Info

Title:
Identification of Biocontrol Strain KC24 and Preliminary Study on the Control Effect of Fermentation Liquid on Begonia Rust
Author(s):
DU Bin; YAN Zhao
(Department of Horticulture,Taiyuan College,Taiyuan,Shanxi 030012)
Keywords:
phylogenetic tree; Paenibacillus polymyxa; Begonia rust; biological control
PACS:
-
DOI:
10.11937/bfyy.20193942
Abstract:
Based on the routine morphological and biochemical detection methods,and combined with 16S rRNA gene sequence analysis and phylogenetic tree identification,a bacterial biocontrol strain was identified as Paenibacillus polymyxa，named KC24.The biological control effect of its fermentation broth was tested on rust.The results showed that the control effect of Paenibacillus polymyxa strain KC24 against Begonia rust was 52.1%,which was equivalent to the control effect of myclobutanil 25% EC (4 000 times).Paenibacillus polymyxa strain KC24 was suitable for bio-controlling begonia rust.

References:

[1]刘鹏远,李厚华,甘林鑫,等.海棠锈病病菌特征观察及其冬孢子萌发培养[J].北方园艺,2018(10):75-81.[2]王淑萍.海棠锈病防治药剂筛选试验[J].现代农业科技,2016(15):111-112.[3]徐志鸿.如何防治海棠锈病[J].绿化与生活,2015(9):17-18.[4]乔洪英.青年湖公园海棠锈病防治的研究[J].北京园林,2014,30(1):50-52.[5]AC〖JX-+0.8mm〗〖KG-*2〗＇ IMOVIC〖JX-+0.8mm〗〖KG-*2〗＇S G，MEREDITH C L.Evaluation of SDHI fungicides and Regalia for control of cedar apple rust in 2016[J].Plant Disease Management Reports,2017,11:PF025.[6]董向丽,李海燕,孙丽娟,等.苹果锈病防治药剂筛选及施药适期研究[J].植物保护,2013,39(2):174-179.[7]马登萍.三唑酮和烯唑醇防治杨树锈病田间药效试验[J].北方园艺,2012(19):157-158.[8]MANTELIN S,BELLAFIORE S,KYNDT T.Meloidogyne graminicola a major threat to rice agriculture[J].Molecular Plant Pathology,2017,18(1):3-15.[9]庞发虎.内生性巨大芽孢杆菌的分离、鉴定及其对小麦叶锈病的生防效果[C].海口：中国植物病理学会2015年学术年会,2015.[10]赵晨晨,焦铸锦,庞发虎,等.生防菌R-4的鉴定及其对玉米南方锈病的防效[J].玉米科学,2017,25(2):136-141.[11]沈萍,范秀荣,李广武.微生物学实验[M].北京:高等教育出版社,1999.[12]权春善,王军华,徐洪涛,等.一株抗真菌解淀粉芽孢杆菌的分离鉴定及其发酵条件的初步研究[J].微生物学报,2006(1):7-12.[13]HONG C，MURUGESAN R，SEK YEE T,et al.Evaluation of five methods for total DNA extraction from western corn root worm beetles[J].PloS One,2010,5(8):e11963.[14]WANG L，WEI Y，HUANG Y.Morganella morganii as a causative agent of disease in the Chinese giant salamander (Andrias davidianus)[J].Asian Herpetological Research,2013,4(3):197-201.[15]WILSON K H，BLITCHINGTON R B，GREENE R C.Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction[J].Journal of Clinical Microbiology,1990,28(9):1942-1946.[16]WANG L，LEE F，TAI C,et al.Comparison of gyrB gene sequences，16S rRNA gene sequences and DNA-DNA hybridization in the bacillus subtilis group[J].International Journal of Systematic and Evolutionary Microbiology,2007,57(8):1846-1850.[17]THOMPSON J D，GIBSON T J，PLEWNIAK F,et al.The CLUSTAL_X windows interface:Flexible strategies for multiple sequencealignment aided by quality analysis tools[J].Nucleic Acids Research，1997,25(24):4876-4882.[18]DARRIBA D，ABOADA G L，OALLO R，et al.Model test 2:More models,new heuristics and high-performance computing[J].Nature Methods,2012,9(8):772.[19]RONQUIST F，HUELSENBECK J P.MrBayes 3:Bayesian phylogenetic inference under mixed models[J].Bioinformatics,2003,19(12):1572-1574.[20]方中达.植病研究方法[M].北京:中国农业出版社,1998.[21]焦振泉,刘秀梅.细菌分类与鉴定的新热点:16S-23SrDNA间区[J].微生物学通报,2001(1):84-89.[22]童蕴慧,郭桂萍,徐敬友,等.拮抗细菌诱导番茄植株抗灰霉病机理研究[J].植物病理学报,2004(6):507-511.[23]史应武,娄恺,李春,等.内生多粘类芽孢杆菌S-7对甜菜光合作用及产量和品质的影响[J].应用生态学报,2009,20(3):597-602.[24]伍明俊,金丹,李晖,等.抗真菌菌株JW-725的分离、鉴定及发酵产物性质的初步分析[J].四川大学学报(自然科学版),2003(5):945-948.[25]文凤云,董淑丽,李晓丽,等.多黏芽孢杆菌Dw-6抗革兰氏阳性菌多肽的分离纯化及抑菌活性机理[J].江苏农业科学,2011,39(3):408-411.[26]姚乌兰,王云山,韩继刚,等.水稻生防菌株多粘类芽孢杆菌WY110抗菌蛋白的纯化及其基因克隆[J].遗传学报,2004(9):878-887.[27]RYU C M，KIM J,CHOI O,et al.Improvement of biological control capacity of Paenibacillus polymyxa E681 by seed pelleting on sesame[J].Biological Control Theory & Application in Pest Management,2006,39(3):282-289.[28]HAO T，CHEN S.Colonization of wheat，maize and cucumber by Paenibacillus polymyxa WLY78[J].PloS One,1932,12(1):e169980.[29]WESELOWSKI B，NATHOO N，EASTMAN A W，et al.Isolation，identification and characterization of Paenibacillus polymyxa CR1 with potentials for biopesticide,biofertilization，biomass degradation and biofuel production[J].Bmc Microbiology,2016,16(1):244.[30]CABANILLAS E，BARKER K R.Impact of Paecilomyces lilacinus inoculum level and application time on control of Meloidogyneincognita on tomato[J].Journal of Nematology,1989,21(1):115-120.

Memo

Memo:
-
Last Update: 2020-08-10