|Table of Contents|

Extraction and Characterization of Lectin From Oudemansiella furfuracea

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年11
Page:
124-132
Research Field:
Publishing date:

Info

Title:
Extraction and Characterization of Lectin From Oudemansiella furfuracea
Author(s):
WANG Jing1; 2; ZHANG Shaonan3; LIU Zhaojun1; CHEN Yuanming4; YAN Xunyou1; 2; CHANG Shimin1; 2
(1.College of Life Sciences，Langfang Normal University，Langfang,Hebei 065000；2.Technical Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province,Langfang,Hebei 065000；3.The 4th Hospital of Hebei Medical University，Shijiazhuang，Hebei 050011；4.China Fire and Rescue Institute，Beijing 102202)
Keywords:
Oudemansiella furfuracea; lectin; double dilution method; agglutinability; hemagglutinating activity; sugarbinding specificity
PACS:
-
DOI:
10.11937/bfyy.20193912
Abstract:
Phosphate buffered solution was used to extract lectin from Oudemansiella furfuracea and double dilution method was carried out to determine thermal stability，salinity tolerance，sugarbinding specificity and agglutinability of erythrocytes，respectively.Single factor experiment and orthogonal experiment were designed to optimize the extraction condition of lectin using hemagglutinating activity as evaluation index to investigate the extraction condition and some characteristics oflectin from Oudemansiella furfuracea.The results showed that lectin from Oudemansiella furfuracea could agglutinate all types of erythrocytes，including rabbit，chick，sheep and human erythrocytes without species specificity or blood type specificity of human，and its agglutinability of rabbit erythrocytes was strongest.Lectin from Oudemansiella furfuracea had a certain thermal stability and salinity tolerance.its agglutinability would not be damaged until the temperature increased to 80 ℃ and effected by phosphate buffered solution containing 0-0.8 mol?L-1 NaCl．Specific sugar of the lectin was N-acetyl-β-D-galactosamine.The optimal extraction condition was obtained as follows，extraction buffer was 0.01 mol?L-1 phosphate buffered solution，the ratio of material to liquid was 1∶15 g?mL-1，the extraction time was 2 hours,pH of extraction buffer was 7.0.

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Last Update: 2020-08-12