|Table of Contents|

Effects of Different Storage Temperature and Time on Viability of Pumpkin Pollens

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年10
Page:
116-121
Research Field:
Publishing date:

Info

Title:
Effects of Different Storage Temperature and Time on Viability of Pumpkin Pollens
Author(s):
LI Qingfei1; 2; WANG Mengmeng1; ZHANG Mannan1; WANG Guangyin1; 2; CHEN Bihua1; 2; LI Xinzheng1; 2
(1.College of Horticulture and Landscape,Henan Institute of Science and Technology,Xinxiang,Henan 453003;2.Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement,Xinxiang,Henan 453003)
Keywords:
pumpkin; pollen viability; temperature; time; assay methods
PACS:
-
DOI:
10.11937/bfyy.20193630
Abstract:
To explore the effects of different storage temperature and time on pumpkin pollens,the pollens of three pumpkin inbred lines were used as materials，and the carmine acetate dyeing,I2-KI and germination in vitro methods were used to determine the viability of pollens,which treated for 0,8,16,24,32 hours at room temperature and treated for 0,24,48,72,96 hours at 4 ℃.The results showed that the pollen viability of pumpkin decreased gradually with time both at room temperature and 4 ℃,as well as the pollen viability decreased rapidly when stored at room temperature,thus 4 ℃ was more suitable for the preservation of pumpkin pollen.Among the three methods,the value of acetic acid magenta method was higher,and the method for pollen germination in vitro was the most reliable.In addition,this study also improved the medium formula of pollen in vitro germination,the germination rate was much higher than previous research.The results would provide a basis for solving the pollination problems of inconsistent male and female flowers and resource with few male flower such as subgynoecious lines.

References:

[1]LU A X,YU M G,FANG Z Y,et al.Preparation of the controlled acid hydrolysates from pumpkin polysaccharides and their antioxidant and antidiabetic evaluation[J].International Journal of Biological Macromolecules,2019,121:261-269.[2]宋丽君.南瓜多糖对糖尿病大鼠血糖、血脂及氧化应激能力的影响[J].中国应用生理学杂志,2015,31(1):65-66.[3]任传军,郝茂钢,马威.籽用南瓜高产栽培技术[J].现代化农业,2011(8):24-25.[4]刘金玉,曹利萍.南瓜不同时间授粉对种子数量与质量的影响[J].山西农业科学,2006(2):47-48.[5]HERRERO M P,JOHNSON R R.High temperature stress and pollen viability of maize[J].Crop Science,1980,20(6):796-800.[6]MESNOUA M,ROUMANI M,SALEM A.The effect of pollen storage temperatures on pollen viability,fruit set and fruit quality of six date palm cultivars[J].Scientia Horticulturae,2018,236:279-283.[7]何晓明,谢大森,彭庆务,等.贮藏温度和时间对节瓜花粉生活力影响的研究[J].广东农业科学,2004(1):21-22.[8]项小燕,吴甘霖,段仁燕,等.不同贮藏温度下大别山五针松花粉活力的变化[J].植物资源与环境学报,2016(2):114-116.[9]石志棉,姬璇,杜勤,等.穿心莲花粉活力测定及离体萌发特性研究[J].广州中医药大学学报,2019,36(4):132-138.[10]董晓莉.连翘花粉活力、花粉形态及细胞培养研究[D].太原:山西大学,2014.[11]梁建萍,韩有志,粱胜发,等.丁香花粉生活力及其贮藏性的研究[J].山西林业科技,2000(1):10-11,19．[12]赵丽丽,李国强,刘爱群,等.贮藏温度对中国南瓜花粉生活力的影响[J].长江蔬菜,2010(10):22-24.[13]许婉芳,蔡英卿,林小兰,等.醋酸洋红染色法测定瓜类花粉的生活力[J].福建果树,2002(S1):7.[14]管雪松,李洪岩,欧阳明安.累积嫁接对黄瓜雌雄花及花粉萌发的影响[J].江苏农业科学,2016,44(6):248-251.[15]FAN L M,WANG Y F,WANG H,et al.In vitro Arabidopsis pollen germination and characterization of the inward potassium currents in Arabidopsis pollen grain protoplasts[J].Journal of Experimental Botany,2001,52(361):1603-1614.[16]叶君营,谢大杰.90-01A苦瓜强雌性系的选育[J].中国蔬菜,1996(4):24.[17]葛长军,闫良,徐丽荣,等.赤霉素对黄瓜雌性系的诱雄效果及诱导性状间的相关性研究[J].湖南农业科学,2016(2):5-8.[18]丁小涛,郝婷,金海军,等.硝酸银和赤霉素处理对黄瓜雌性系诱雄效果的比较[J].上海交通大学学报(农业科学版),2013,31(6):1-5.[19]SUSIACUE I,JOS M .Fertility and pollen tube growth in polyploid melons (Cucumis melo L.)[J].Euphytica,1997,93(3):369-373.[20]管雨,贾文庆,刘会超,等.木瓜花粉生活力测定及储藏特性[J].浙江农林大学学报,2012,29(5):790-794.[21]胡新军,粟建文,袁祖华,等.苦瓜花粉体外萌发培养基成分的优化[J].湖南农业大学学报(自然科学版),2009(1):69-72.[22]郭军洋,陈龙正,曹清河,等.黄瓜现采花粉生活力最佳染色方法的筛选[J].广东农业科学,2004(6):48-49.

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Last Update: 2020-07-25