|Table of Contents|

Genetic Diversity Analysis of Amomum tsao-ko in Main Producing Area Using RAPD Markers

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年10
Page:
121-127
Research Field:
Publishing date:

Info

Title:
Genetic Diversity Analysis of Amomum tsao-ko in Main Producing Area Using RAPD Markers
Author(s):
MA MengliZHANG WeiMENG HenglingWANG TiantaoZHANG TingtingLU Bingyue
(College of Life Science and Technology,Honghe University/Key Laboratory of Biological Resources Research and Utilization in Southern Yunnan,Mengzi,Yunnan 661100)
Keywords:
Amomum tsao-kogenetic diversityRAPD markersgenetic differentiation
PACS:
-
DOI:
10.11937/bfyy.20193422
Abstract:
The genetic diversity of 48 Amomum tsao-ko from four populations in Honghe Prefecture of Yunnan Province was analyzed by random amplified polymorphic DNA (RAPD) molecular marker technique.This study could provide an important theoretical basis for the protection and utilization of A.tsao-ko resources.The results showed that a total of 139 bands were amplified by 11 RAPD primers,and the ratio of polymorphic bands (PPB) was 98.56%.At the population level,Jinping population had the highest genetic diversity (PPB=82.01%),followed by Lvchun population (PPB=66.91%),Pingbian population (57.55%) and Yuanyang population (55.40%).87.67% (Hs=0.197) of the total genetic diversity of A.tsao-ko came from within populations,and the genetic variation among populations accounted for only 12.33%(Gst=0.123),analysis of molecular variance (AMOVA) further proved that the genetic variation of A.tsao-ko mainly existed within populations.Four A.tsao-ko populations shared high levels of genetic identity (0.931 4-0.971 7),and the genetic variation among the populations was small.

References:

[1]LIM T K.Amomum tsao-ko,In edible medicinal and non-medicinal plants[M].Berlin:Springer Netherlands Press,2013.[2]LIU G,JIN M,CAI C,et al.Soil microbial community structure and physicochemical properties in Amomum tsao-ko based agroforestry systems in the Gaoligong Mountains,Southwest China[J].Sustainability,2019,11(2):546.[3]SHI Y N,JIN H,YANG Y,et al.Herbal verification of medicinal Amomum tsao-ko[J].Med Plant,2014,5(9):16-19,24.[4]杨绍兵,张金渝.草果生产加工适宜技术[M].北京:中国医药科技出版社,2018.[5]张拓.人工合成小麦遗传多样性及重要农艺性状变异分析[D].杨凌:西北农林科技大学,2017.[6]WILLIAMS J G K,KUBELIK A R,LIVAK K J,et al.DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J].Nucleic Acids Research,1990,18(22):6531-6535.[7]SHARMA S,KUMAR P,GAMBHIR G,et al.Assessment of genetic diversity in lettuce (Lactuca sativa L.) germplasm using RAPD markers[J].3 Biotech,2018,8(1):9.[8]KAUR P,PANDEY D K,GUPTA R C,et al.Assessment of genetic diversity among different population of five Swertia species by using molecular and phytochemical markers[J].Industrial Crops and Products,2019,138:111569.[9]PIDIGAM S,MUNNAM S B,NIMMARAJULA S,et al.Assessment of genetic diversity in yardlong bean (Vigna unguiculata (L.) Walp subsp.sesquipedalis Verdc.) germplasm from India using RAPD markers[J].Genetic Resources and Crop Evolution,2019,66(6),1231-1242.[10]LIU H,YAN Q,ZOU D,et al.Identification and bioactivity evaluation of ingredients from the fruits of Amomum tsaoko Crevost et Lemaire[J].Phytochemistry Letters,2018,28:111-115.[11]SIM S,TAN S K,KOHLENBERG B,et al.Amomum tsao-ko:Chinese Black Cardamom:Detailed oil composition and comparison with two other Cardamom species[J].Natural Product Communications,2019,14(7):1934578X19857675.[12]KIM J G,JANG H,LE T P L,et al.Pyranoflavanones and pyranochalcones from the fruits of Amomum tsao-ko[J].Journal of Natural Products,2019,82(7):1886-1892.[13]YANG Y W,YANG Z Y,YAN M R,et al.Isolation and characterization of microsatellite markers for Amomum tsaoko (Zingiberaceae),an economically important plant in China[J].Genetics and Molecular Research,2014,13(4):8220-8224.[14]谢正万,宁德鲁,周军,等.适于草果遗传多样性分析的RAPD引物筛选[J].西部林业科学,2018,47(4):45-50.[15]胡一凡,张雪梅,徐绍忠,等.云南草果种质资源的遗传多样性及亲缘关系的SSR分析[J].中草药,2018,49(22):163-170.[16]PEAKALL R O D,SMOUSE P E.GENALEX 6:Genetic analysis in excel.Population genetic software for teaching and research[J].Molecular Ecology Notes,2006,6(1):288-295.[17]ROBARTS D W H,WOLFE A D.Sequence-related amplified polymorphism (SRAP) markers:A potential resource for studies in plant molecular biology[J].Applications in Plant Sciences,2014,2(7):1400017.[18]徐吉银,丁平.道地药材阳春砂不同居群的RAPD分析[J].中药新药与临床药理,2005,16(3):194-196.[19]张忠廉,李学兰,杨春勇,等.砂仁遗传多样性的ISSR分析[J].中草药,2011,42(3):570-574.[20]BOOY G,HENDRIKS R J J,SMULDERS M J M,et al.Genetic diversity and the survival of populations[J].Plant Biology,2000,2(4):379-395.[21]NYBOM H.Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants[J].Molecular Ecology,2004,13(5):1143-1155.[22]LI M,CHEN X,HUANG M,et al.Genetic diversity and relationships of ancient Chinese fir (Cunninghamia lanceolata) genotypes revealed by sequence-related amplified polymorphism markers[J].Genetic Resources and Crop Evolution,2017,64(5):1087-1099.[23]陈宗游,黄夕洋,唐辉,等.广西甜茶种质资源遗传多样性的ISSR分析[J].园艺学报,2017,44(1):161-169.[24]姜武,吴志刚,陶正明,等.基于ISSR和SRAP标记的栀子种质遗传多样性研究[J].中草药,2019,50(2):251-257.[25]HAMRICK J L,GODT M J W.Effects of life history traits on genetic diversity in plant species[J].Philosophical Transactions of the Royal Society of London.Series B:Biological Sciences,1996,351(1345):1291-1298.[26]BUSSELL J.The distribution of random amplified polymorphic DNA (RAPD) diversity amongst populations of Isotoma petraea (Lobeliaceae)[J].Molecular Ecology,1999(8):775-789.[27]崔晓龙,魏蓉城,黄瑞复.草果遗传体系的初步研究[J].云南大学学报(自然科学版),1995,17(3):290-297.[28]李国栋,张洪武,刘小莉,等.草果两型植株的繁育差异研究[J].广西植物,2017,37(10):1312-1321.

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Last Update: 2020-07-26