|Table of Contents|

Expression，Purification and Crystallization of Carnitine Acyl Transferase From Phytophthora capsica

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年11
Page:
42-46
Research Field:
Publishing date:

Info

Title:
Expression，Purification and Crystallization of Carnitine Acyl Transferase From Phytophthora capsica
Author(s):
WANG Huizheng; LAN Yubin
(College of Agricultural Engineering and Food Science，Shandong University of Technology/National Sub-center for International Collaboration Research on Precision Agricultural Aviation Pesticide Spraying Technology，Zibo,Shandong 255000)
Keywords:
Phytophthora capsici; carnitine acyl transferase; expression; purification; crystallization
PACS:
-
DOI:
10.11937/bfyy.20193355
Abstract:
Carnitine acyl transferase CAT is a kind of enzymes closely related to appressorium formation and pathogenicity of Phytophthora capsici.Based on the whole-genome sequence data of P.capsici，the whole genome DNA of highly pathogenic strain P.capsici SD33 was extracted and used to amplify CAT gene.The recombinant protein 6×His-CAT was induced and expressed by prokaryotic expression method，and the recombinant protein was purified by affinity chromatography，molecular exclusion chromatography.The crystallization experiment using purified protein was carried out in order to obtain protein crystals.The results showed that the protein obtained by the above method had high purity and good crystallinity，provided a basis to clarify the mechanism of CAT.

References:

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Last Update: 2020-08-12