|Table of Contents|

Effects of Temperature，Gibberellin Acid(GA3) and KNO3 Solution Presoaking on Seed Germination of Ilex pubescens

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年11
Page:
112-116
Research Field:
Publishing date:

Info

Title:
Effects of Temperature，Gibberellin Acid(GA3) and KNO3 Solution Presoaking on Seed Germination of Ilex pubescens
Author(s):
TONG Jiayun; LI Yunxuan; HUANG Huan; LIN Shaodong; HUANG Aihua
(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510006)
Keywords:
Ilex pubescens; seed germination; temperature; GA3; KNO3
PACS:
-
DOI:
10.11937/bfyy.20193056
Abstract:
To seek a proper temperature for Ilex pubescens seeds germination and find an optimal concentration of gibberellin acid(GA3) or potassium nitrate（KNO3） for seeds pretreatment.After stored with wet sand at 4 ℃ for 71 days,seeds were set up in 8 groups and sowed in petri dishes.There were 3 groups for temperature investigation,20 ℃,25 ℃ and 30 ℃.And 5,10,20 g?L-1 GA3,5 g?L-1 KNO3,mixed solution of 5 g?L-1 KNO3 and 10 g?L-1 GA3,and distilled water were used to pre-soak the seeds respectively.Parameters of germination percentage,germination energy,germination index,fresh weight of seedlings,vigor index,lengths of radical and hypocotyl were used to investigate the effects.There was no significant difference of the germination percentages between the groups at three temperatures；germination energy and germination index of seeds at 20 ℃ were significantly lower than those in 25 ℃.Vigor index of seeds at 30 ℃ was significantly lower than those in 25 ℃.After soaking seed with 10 g?L-1GA3,the germination energy and germination index were significantly higher than the control group.Conclusion the optimal germination temperature was 25 ℃,and 10 g?L-1 GA3 was suitable for pretreatment of Ilex pubescens seeds before germination.

References:

[1]国家中医药管理局中华本草编委会.中华本草.第五册[M].上海：上海科技出版社，1999：155-159.[2]CHEN S K,MA H Y,FENG Y X,et al.Flora of China.vol.11[M].Bejing：Science Press,2008：419-420.[3]GALINDEZ G,CECCATO D,BUBILLO R,et al.Three levels of simple morphophysiological dormancy in seeds of Ilex (Aquifoliaceae) species from Argentina[J].Seed Science Research,2018,28(2)：1-9.[4]贾有伟，魏驰宇，田如男.欧洲冬青种皮特性与种子休眠的关系[J].江苏农业科学，2017,45(12)：96-99.[5]ARRIETA S,SUREZ F.Germination and seed bank depletion of holly (Ilex aquifolium L.) in four microhabitat types[J].Seed Science Research,2004,14(3)：305-313.[6]徐本美，史晓华，孙运涛，等.大果冬青种子的休眠与萌发初探[J].种子，2002,21(3)：1-2,5.[7]徐本美，史晓华，黎念林.大叶冬青种子的休眠与萌发初探[J].植物引种驯化集刊，1997(11)：150.[8]周晓峰，彭方仁.双核冬青种子休眠原因与萌发特性[J].林业科技开发，2010,24(2)：34-38.[9]何彦峰.狭叶冬青种子休眠与萌发的研究[J].浙江林业科技,2008,28(3)：63-65.[10]BETHKE P C,LIBOURELI G L,AOYAMA N,et al.The Arabidopsis aleurone layer responds to nitric oxide,gibberellin,and abscisic acid and is sufficient and necessary for seed dormancy[J].Plant Physiology,2007,143：1173-1188.[11]中华人民共和国国家标准GB/T 3543.4—1995.农作物种子检验规程[S].北京：中国标准出版社，1995.[12]支巨振．《农作物种子检验规程》实施指南[M]．北京：中国标准出版社，2000：43-51.[13]MARN J A,ANDREU P,CARRASCO A,et al.Determination of proline concentration,an abiotic stress marker,in root exudates of excised root cultures of fruit tree rootstocks under salt stress[J].Revue des Régions Arides,2010,24：722-727.[14]陈建勋，王晓峰.植物生理学实验指导[M].2版.广州：华南理工大学出版社，2006：66-67.[15]ASHRAF M,FOOLAD M.Roles of glycine betaine and proline in improving plant abiotic stress resistance[J].Environmental and Experimental Botany,2007,59(2)：206-216.[16]HOLDSWORTH M J,BENTSINK L,SOPPE W J J.Molecular networks regulating Arabidopsis seed maturation,after-ripening,dormancy and germination[J].New Phytologist,2008,179(1)：33-54.[17]王利英.几种浙江省乡土冬青属植物繁殖技术和生物学生态学特性研究[D].杭州：浙江林学院,2008.[18]曾庆钱，杨红梅，黄珊珊，等.梅叶冬青种子的发芽特性研究[J].种子，2012,31(2)：78-80.

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Last Update: 2020-08-12