|Table of Contents|

Establishment and Optimization of AFLP Molecular Marker Technology System of Meconopsis punicea

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年09
Page:
131-139
Research Field:
Publishing date:

Info

Title:
Establishment and Optimization of AFLP Molecular Marker Technology System of Meconopsis punicea
Author(s):
OU Zhi; YAN Pengfei; ZHANG Yingxin; JIA Weijia; QU Yan
(College of Landscape Architecture and Horticulture Sciences,Southwest Forestry University/Southwest Landscape Architecture Engineering Research Center of State Forestry Administration/Provincial Key Laboratory of Ornamental Plants and Horticulture,Kunming,Yunnan 650224)
Keywords:
Meconopsis punicea; AFLP; system of establishment and optimization
PACS:
-
DOI:
10.11937/bfyy.20192991
Abstract:
The Meconopsis puniceawas used as template in this study.Used orthogonal experimental design,the effect of relevant factors such as the digestion time,the amount of Mg2+,the amount of dNTPS,and the template DNA dilution factor on the AFLP reaction system were studied.The goal was to establish an AFLP reaction system suitable for M.punicea and screen 64 pairs of AFLP primers with the optimized system.The results showed that the clear bands could be obtained through the established AFLP reaction system,and 8 pairs of primer combinations suitable for AFLP analysis of M.punicea were screened by using this system.This study provided a certain reference for future genetic diversity analysis and germplasm identification of AFLP markers in M.Vig.

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Last Update: 2020-07-24