|Table of Contents|

Enzyme-Assisted Extraction of Total Flavonoids From Bidens bipinnata L.Using Response Surface Methodology

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年11
Page:
104-111
Research Field:
Publishing date:

Info

Title:
Enzyme-Assisted Extraction of Total Flavonoids From Bidens bipinnata L.Using Response Surface Methodology
Author(s):
YANG Xiudong; BAI Zifan; CHEN Xin; HAN Jia; MIAO Bingtao; ZHOU Hongli
(College of Chemistry and Pharmaceutical Engineering,Jilin Institute of Chemical Technology,Jilin,Jilin 132022)
Keywords:
Bidens bipinnata L.; total flavonoids; enzyme-assisted; extraction process; response surface method
PACS:
-
DOI:
10.11937/bfyy.20192947
Abstract:
Bidens bipinnata L.was used as test material.The enzyme-assisted extraction process of total flavonoids from B.bipinnata L.was investigated and optimized by response surface method.The effects of enzyme type,enzyme dosage,hydrolysis time,ethanol concentration,hydrolysis temperature and pH on the extraction rate of total flavonoids from B.bipinnata L.were investigated.The in vitro antioxidant activity of the extract from B.bipinnata L.was also studied.The results showed that pectinase was the best enzyme type,and the optimum extraction conditions were as follows hydrolysis time 2 hours,ethanol concentration 63%,enzyme hydrolysis pH 4,the predicted value was 2.07%.Under these conditions,the extraction rate of total flavonoids from B.bipinnata L.was 1.95%.The IC50 values of total flavonoids extract from B.bipinnata L.against DPPH,ABTS and superoxide anion free radicals were 0.172 9,0.253 7 and 0.564 3,respectively.Therefore,the study suggested that the process of pectinase assisted extraction was stable and feasible.The results could provide a scientific basis for developing and utilization as a natural antioxidant of total flavonoids from B.bipinnata L..

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Last Update: 2020-08-12