|Table of Contents|

Isolation and Identification of Fungal Disease in Cordyceps militaris Cultivation

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年04
Page:
129-134
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Fungal Disease in Cordyceps militaris Cultivation
Author(s):
LIU Guoli; LI Hong; ZHANG Min
(Institute of Edible Fungi,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161)
Keywords:
Cordyceps militaris; disease; fungi; isolation and identification
PACS:
-
DOI:
10.11937/bfyy.20192894
Abstract:
In order to identify the species of contaminated fungi in cultured Cordyceps militaris medium,the pathogenic fungi was separated by streak plate method,and morphological identification and ITS sequence phylogenetic analysis were carried out.The results showed that three fungi strains were isolated from the contaminated culture material during C.militaris cultivation.P1 and P5 isolates had the highest homology with several Trichoderma atroviride isolates in GenBank,all of which were more than 99.6%.Combined with morphological characteristics,P1 and P5 isolates were identified as T.atroviride.The strain P3 had the highest homology with Penicillium expansum isolates in GenBank,and combined with morphological characteristics,P3 was identified as P.expansum.The results provided a theoretical basis for the identification and control of the fungus diseases of C.militaris cultivation.

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Last Update: 2020-03-30