|Table of Contents|

Optimization of Subculture Medium for Tissue Culture of Epimedium brevicornu Maxim.in Danjiangkou

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年08
Page:
108-115
Research Field:
Publishing date:

Info

Title:
Optimization of Subculture Medium for Tissue Culture of Epimedium brevicornu Maxim.in Danjiangkou
Author(s):
DAI LeiYANG ZhenJIAN ZaiyouZHOU XiurenXU GuifangMENG Li
(College of Life Science and Technology, Henan Institute of Science and Technology,Xinxiang,Henan 453003)
Keywords:
Epimedium brevicornu Maxim.tissue cultureresponse surface methodsubculture medium
PACS:
-
DOI:
10.11937/bfyy.20192763
Abstract:
Taking Epimedium brevicornu Maxim.in Danjiangkou as materials,the effects of different concentrations of 6-BA,NAA and sucrose in tissue culture medium on the growth of Epimedium brevicornu Maxim.were compared by using the method of single factor experiment and response surface analysis,in order to provide reference for factory production of Eimedium brevicoru.The results showed that these three factors had significant effects on the growth of Epimedium brevicornu Maxim..On the basis,the induced shoot bud subculture medium was optimized by response surface methodology,and the propagating effect of Epimedium brevicornu Maxim.was better,when the concentration of NAA was 0.56 mg?L-1,the concentration of sucrose was 31.47 g?L-1,and the concentration of 6-BA was 2.03 mg?L-1 in the subculture medium,and the growth score could reach 84.88.Therefore,the subculture medium could improve the coefficient of fertility of Epimedium brevicornu Maxim.,which could solve the problem that the wild-type proliferative ability of Epimedium brevicornu Maxim.was weak and the wild seedlings were extremely difficult to survive.

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Last Update: 2020-07-08